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Related Experiment Video

Updated: Jul 15, 2026

Rare Event Detection Using Error-corrected DNA and RNA Sequencing

Rare Event Detection Using Error-corrected DNA and RNA Sequencing

Published on: August 3, 2018

Pilot study: assessment of interlaboratory variability of sequencing-based typing DNA sequence data quality.

D C Sayer¹, D M Goodridge

¹Conexio Genomics, Fremantle, Western Australia, Australia. david@conexio.iinet.net.au

Tissue Antigens

|April 21, 2007

Summary

Sequence quality scores for human leukocyte antigen typing by sequence-based typing (SBT) vary between labs. The Assign-SBT base call score (BCS) system effectively compares this sequence quality across different laboratories.

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Last Updated: Jul 15, 2026

Rare Event Detection Using Error-corrected DNA and RNA Sequencing

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Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.

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Comparative Lesions Analysis Through a Targeted Sequencing Approach

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Published on: November 5, 2019

Area of Science:

Immunogenetics
Molecular Biology
Clinical Diagnostics

Background:

Human leukocyte antigen (HLA) typing is crucial for organ transplantation and disease association studies.
Sequence-based typing (SBT) is a common method for high-resolution HLA allele identification.
Ensuring consistent sequence quality across different laboratories is essential for reliable HLA typing results.

Purpose of the Study:

To assess the variability of sequence quality scores in human leukocyte antigen typing using sequence-based typing (SBT) across different laboratories.
To evaluate the utility of the Assign-SBT base call score (BCS) system for inter-laboratory sequence quality comparison.

Main Methods:

Pilot study involving multiple laboratories performing human leukocyte antigen typing.
Utilized sequence-based typing (SBT) methodology for HLA allele identification.
Analyzed sequence quality scores and applied the Assign-SBT base call score (BCS) system.

Main Results:

Significant variability in sequence quality scores was observed both within and between participating laboratories.
The base call score (BCS) system demonstrated effectiveness in quantifying and comparing sequence quality across different laboratory settings.
Identified a need for standardized quality metrics in sequence-based HLA typing.

Conclusions:

Sequence quality in human leukocyte antigen typing by sequence-based typing (SBT) is not uniform across laboratories.
The Assign-SBT base call score (BCS) system provides a valuable tool for monitoring and standardizing sequence quality in multi-laboratory SBT workflows.
Implementing BCS-based quality control can enhance the reliability and comparability of HLA typing results globally.