Related Experiment Video
Updated: Jul 15, 2026
08:27
Detection of Human Leukocyte Antigen Biomarkers in Breast Cancer Utilizing Label-free Biosensor Technology
Published on: March 24, 2015
HLA-G levels in serum and plasma.
N Rudstein-Svetlicky1, R Loewenthal, V Horejsi
1TissueTyping Laboratory, Sheba Medical Center and Tel Aviv University, Tel Aviv, Israel.
Tissue Antigens
|April 21, 2007
Summary
Soluble human leukocyte antigen (HLA)-G is typically higher in plasma than serum due to clot formation. Researchers should compare plasma to plasma and serum to serum for accurate HLA-G measurements.
Area of Science:
- Immunogenetics
- Biochemistry
Background:
- Soluble human leukocyte antigen (HLA)-G plays a role in immune regulation.
- Accurate quantification of soluble HLA-G is crucial for various research applications.
Purpose of the Study:
- To investigate the discrepancy in soluble HLA-G levels between plasma and serum.
- To provide recommendations for standardized measurement of soluble HLA-G.
Main Methods:
- Comparison of soluble HLA-G levels in simultaneously collected plasma and serum samples.
- Analysis of potential factors influencing soluble HLA-G quantification.
Main Results:
- Soluble HLA-G levels were consistently higher in plasma compared to serum from the same individuals.
- Clot formation during serum processing appears to trap soluble HLA-G.
- Potential gender-based differences in soluble HLA-G levels were observed.
Conclusions:
- Standardized protocols are necessary for soluble HLA-G quantification, comparing plasma to plasma and serum to serum.
- Control groups in studies should account for potential gender differences in soluble HLA-G levels.
