Related Experiment Video
Updated: Jul 15, 2026
A 3D Human Lung Tissue Model for Functional Studies on Mycobacterium tuberculosis Infection
Published on: October 5, 2015
[The role of CD8+CD28- regulatory T lymphocytes in pulmonary tuberculosis]
Tao Yu1, Yu-hao Yang, De-qiong Dong
1Department of Respiratory Disease Affiliated Hospital of Zunyi Medical College, Guizhou 563003, China.
Objective:
To study the role of CD(8)(+)CD(28)(-) regulatory T cells in tuberculosis.
Methods:
The positive rates of CD(3)(+)CD(8)(+)CD(28)(-) T cells, CD(3)(+) T cells, CD(3)(+)CD(8)(+) T cells and CD(8)(+)CD(28)(+) T cells, and the content of IL-6 in CD(8)(+)CD(28)(-) T cells in leukocytes of peripheral blood from 15 patients with pulmonary tuberculosis, 15 patients with chronic bronchitis and 15 healthy controls were detected by flow cytometry.
Results:
The positive expression rates of CD(3)(+) T cells of tuberculosis group [(41 +/- 16)%] and bronchitis controls [(40 +/- 10)%] were significantly lower than those from healthy controls [(44 +/- 6)%] respectively, but no differences were found between tuberculosis group and bronchitis controls. The CD(3)(+)CD(8)(+) T cells in CD(3)(+) T cells from the tuberculosis group [(47 +/- 16)%] and bronchitis controls [(44 +/- 10)%] were significantly higher than those from the healthy controls [(41 +/- 12)%] respectively, but no differences were found between the tuberculosis group and bronchitis controls. The CD(8)(+)CD(28)(+) T cells in CD(3)(+) T cells from tuberculosis group [(15 +/- 8)%] and bronchitis controls (20 +/- 7%) were significantly lower than those from healthy controls [(32 +/- 9)%] respectively, and those of the tuberculosis group were significantly lower than those from the bronchitis controls. CD(8)(+)CD(28)(-) T cells in CD(3)(+) T cells from tuberculosis group [(27 +/- 9)%] and bronchitis controls [(22 +/- 9)%] were significantly higher than those from healthy controls [(10 +/- 4)%] respectively, and those of the tuberculosis group were significantly higher than those of the bronchitis controls. The level of IL-6 secreted by CD(8)(+)CD(28)(-) T cells from tuberculosis group [(32.4 +/- 2.4)%] was significantly higher than bronchitis controls [(19.7 +/- 3.2)%] and healthy controls [(15.2 +/- 2.7)%] and no differences were found between bronchitis controls and healthy controls.
Conclusions:
The number of CD(8)(+)CD(28)(-) T cells and their production of IL-6 in the peripheral blood of tuberculosis patients are significantly increased as compared with the control groups, while the number of CD(8)(+)CD(28)(+) T cells (cytotoxic T cell) is significantly decreased. The results suggest that these cells and IL-6 may be involved in the pathogenesis of pulmonary tuberculosis.
Related Concept Videos
01:28Pulmonary Tuberculosis V
Latent tuberculosis infection occurs when TB bacteria are present in a person's body, but are not causing illness or symptoms. It is not contagious, and preventive treatment is crucial to avoid the progression...
01:28Pulmonary Tuberculosis II
Here is a detailed explanation of its pathophysiology:
Transmission: The process begins when a person inhales droplet nuclei containing M. tuberculosis. These are typically released into the air when an individual with pulmonary or...
01:29Pulmonary Tuberculosis I
Causative Organism
The primary infectious agent causing tuberculosis is Mycobacterium tuberculosis, a slow-growing, acid-fast, aerobic rod that exhibits sensitivity to heat and ultraviolet light. Instances of Mycobacterium bovis and Mycobacterium avium contributing to the development of TB infection are rare.
Mode of...
01:23Tuberculosis
01:24T Cell Types and Functions
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
01:26Pulmonary Tuberculosis IV
Several diagnostic approaches are used to detect TB. The conventional method is the Tuberculin Skin Test (TST), also known as the Mantoux test. However, this method has...