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Purification of Mitochondria from Yeast Cells
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Purification of Mitochondria from Yeast Cells

Published on: August 24, 2009

Purification and subfractionation of mitochondria from the yeast Saccharomyces cerevisiae

Istvan R Boldogh1, Liza A Pon

  • 1Department of Anatomy and Cell Biology, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA.

Methods in Cell Biology
|April 21, 2007
PubMed
Abstract

No abstract available in PubMed .

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Subcellular Fractionation01:32

Subcellular Fractionation

The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
Differential centrifugation is...

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