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Updated: Jul 15, 2026
13:56
A Comparative Approach to Characterize the Landscape of Host-Pathogen Protein-Protein Interactions
Published on: July 18, 2013
Epstein-Barr virus and virus human protein interaction maps.
Michael A Calderwood1, Kavitha Venkatesan, Li Xing
1Program in Virology, Department of Medicine, The Channing Laboratory, Harvard Medical School and Brigham and Women's Hospital, Boston, MA 02115, USA.
Summary
This study maps Epstein-Barr virus (EBV) protein interactions, revealing 43 EBV-EBV and 173 EBV-human protein connections. These interactome maps offer insights into EBV
Area of Science:
- Virology
- Molecular Biology
- Systems Biology
Background:
- Epstein-Barr virus (EBV) is a human herpesvirus implicated in various cancers.
- Understanding EBV protein interactions is crucial for elucidating its replication and persistence strategies.
Purpose of the Study:
- To comprehensively map interactions among EBV proteins and between EBV and human proteins.
- To generate hypotheses regarding EBV's functional mechanisms and host cell manipulation.
Main Methods:
- Utilized a stringent high-throughput yeast two-hybrid system.
- Systematically assessed protein-protein interactions for EBV and human proteins.
Main Results:
- Identified 43 EBV-EBV and 173 EBV-human protein interactions.
- EBV-EBV interactions were enriched within conserved or subfamily-specific gene classes.
- EBV proteins preferentially target highly connected human 'hub' proteins.
Conclusions:
- The EBV interactome provides a framework for understanding viral strategies.
- Targeting human hub proteins may be an efficient mechanism for EBV to reorganize cellular processes.
- The study identified a novel interaction where EBV protein LF2 inhibits Rta transactivation.
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