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Updated: Jul 15, 2026

Isolation of Human Myoblasts, Assessment of Myogenic Differentiation, and Store-operated Calcium Entry Measurement
Published on: July 26, 2017
Three types of muscles express three sodium-calcium exchanger isoforms
1Université de Nantes, CNRS UMR 6204, Biotechnologie, Biocatalyse et Biorégulation, Faculté des Sciences et des Techniques, 44322 Nantes Cedex 3, France. Dmitri.Levitsky@univ-nantes.fr
Abstract:
The sodium-calcium exchanger (NCX) of plasma membrane is expressed in any animal cell. The specific role of its three isoforms (NCX1-3) is not yet established. Their levels vary considerably during murine postnatal development. In particular, in skeletal muscle, NCX1 expression decreases gradually upon aging while reciprocal changes take place for NCX3. NCX2 expression is restricted to brain and smooth muscles. The data on SDS-gel mobility shifts indicate that all three isoforms undergo Ca2+-dependent conformational changes, and that an exchanger regulatory Ca2+-binding domain interacts directly with mutually exclusive exons A and B inducing two different NCX1 conformations.
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