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Published on: November 17, 2010
Enhanced gene transfer into brain capillary endothelial cells using Antp-modified DNA-loaded nanoparticles
Rong-Qin Huang1, Yuan-Ying Pei, Chen Jiang
1Department of Pharmaceutics, School of Pharmacy, Fudan University, P.O. Box 232, Shanghai, 200032, China.
Journal of Biomedical Science
|April 21, 2007
Summary
Antennapedia peptide (Antp) modification enhances DNA nanoparticle delivery to brain capillary endothelial cells. This improves gene transfection and expression, offering a novel non-viral gene vector for cerebral gene therapy.
Area of Science:
- Biomedical Engineering
- Gene Therapy
- Nanotechnology
Background:
- Brain capillary endothelial cells (BCECs) are key targets for cerebral gene therapy.
- BCECs exhibit poor endocytosis, hindering transfection with conventional non-viral vectors.
Purpose of the Study:
- To enhance DNA/polymer nanoparticle transfection and expression efficiency in BCECs.
- To investigate the role of Antennapedia peptide (Antp) modification in improving gene delivery.
Main Methods:
- DNA-loaded nanoparticles were prepared using polyethylenimine (PEI) or polyamidoamine (PAMAM).
- Nanoparticles were modified with Antp using a complex coacervation technique.
- Transfection efficiency and gene expression in BCECs were evaluated.
Main Results:
- Antp modification significantly enhanced transfection and expression efficiency for both DNA/PEI and DNA/PAMAM nanoparticles.
- DNA/Antp/PEI nanoparticles showed improved gene expression without increased cellular uptake after 3 hours.
- DNA/Antp/PAMAM nanoparticles demonstrated enhanced transfection and expression.
Conclusions:
- Antp modification improves the membrane-penetrating ability of DNA-loaded nanoparticles.
- Antp-modified nanoparticles represent promising novel non-viral gene vectors for cerebral applications.
