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Isolation and Quantification of Epstein-Barr Virus from the P3HR1 Cell Line
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Diagnostic evaluation of a new panel of assays for Epstein-Barr virus serology

M Radtke1, B Gärtner, D Franke

  • 1Medac GmbH, Diagnostic Division, Hamburg, Germany. m.radtke@medac.de

Clinical Laboratory
|April 24, 2007
PubMed
Abstract

No abstract available in PubMed .

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Enzyme-Linked Immunosorbent Assay01:33

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In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.

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