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Updated: Jul 15, 2026

Biosynthesis of a Flavonol from a Flavanone by Establishing a One-pot Bienzymatic Cascade
Published on: August 14, 2019
Combinatorial mutasynthesis of flavonoid analogues from acrylic acids in microorganisms
Joseph A Chemler1, Yajun Yan, Effendi Leonard
1State University of New York at Buffalo, Department of Chemical and Biological Engineering, 303 Furnas Hall, Buffalo, NY 14260, USA.
Abstract:
Flavonoids are plant secondary metabolites often used as nutraceutical supplements, but a growing number of unnatural flavonoids are being investigated as therapeutic agents. Cultures of Saccharomyces cerevisiae expressing recombinant flavonoid enzymes, including 4-coumaroyl:CoA ligase (4CL), chalcone synthase (CHS), chalcone isomerase (CHI), and flavanone 3beta-hydroxylase (FHT), produced novel flavanones and dihydroflavonols when fed with a number of aromatic acrylic acids. The flavonoid network also exhibited broad substrate specificity by converting muconic acid into a unique polypropanoid.

