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On the role of 26-deoxylaidlomycin in laidlomycin biosynthesis
Journal of Basic Microbiology
|January 1, 1991
Abstract:
3H-26-deoxylaidlomycin (I) was fed to growing cultures of Streptoverticillium olivoreticuli IMET 43782 producing both I and laidlomycin (II). The incorporation of labelled I into laidlomycin II was neglible showing a role of I as precursor of II to be at variance. Otherwise, a measurable conversion was achieved by the use of sonicated 48 hrs mycelium suggesting that final oxidation of I to II could be mediated by a compartmentized oxidoreductase.
Insights
3H-26-deoxylaidlomycin was not a direct precursor to laidlomycin II in Streptoverticillium olivoreticuli. Final oxidation may involve a compartmentized oxidoreductase.
Area of Science:
- Microbiology
- Biochemistry
- Molecular Biology
Background:
- Streptoverticillium olivoreticuli producesaidlomycin (I) and laidlomycin (II).
- The biosynthetic relationship betweenaidlomycin I and laidlomycin II requires elucidation.
Purpose of the Study:
- To investigate the role of 3H-26-deoxylaidlomycin (I) as a precursor to laidlomycin (II) in Streptoverticillium olivoreticuli.
- To explore the mechanism of conversion fromaidlomycin I to laidlomycin II.
Main Methods:
- Culturing Streptoverticillium olivoreticuli IMET 43782.
- Feeding experiments with radiolabeled 3H-26-deoxylaidlomycin (I).
- Analysis of laidlomycin (II) incorporation using radioactivity detection.
- Utilizing sonicated mycelium for conversion studies.
Main Results:
- Negligible incorporation of labeledaidlomycin (I) into laidlomycin (II) was observed in growing cultures.
- Measurable conversion ofaidlomycin (I) to laidlomycin (II) was achieved using sonicated mycelium.
- These findings suggestaidlomycin I is not a direct precursor to laidlomycin II.
Conclusions:
- The direct precursor role ofaidlomycin (I) to laidlomycin (II) is unlikely.
- A compartmentized oxidoreductase may mediate the final oxidation step fromaidlomycin (I) to laidlomycin (II).