Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Marked gene transcript level alterations occur early during radical prostatectomy.

Thorsten Schlomm1, Esther Näkel, Andreas Lübke

  • 1Martini-Clinic, Prostate Cancer Center, University Medical Center, Hamburg-Eppendorf, Germany. tschlomm@uke.uni-hamburg.de

European Urology
|April 24, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Towards clinically interpretable machine learning in emergency surgery: feature importance and insights across clinical time points in abdominal pain cases.

Langenbeck's archives of surgery·2026
Same author

[Ophthalmic pathology grossing: The globe].

Pathologie (Heidelberg, Germany)·2026
Same author

miRNA profiling in pediatric and young adult Burkitt leukemia and lymphoma.

Virchows Archiv : an international journal of pathology·2026
Same author

Drug-Induced Liver Failure Following Topical Minoxidil: A Case Report.

Case reports in gastrointestinal medicine·2025
Same author

Pathologist-like explainable AI for interpretable Gleason grading in prostate cancer.

Nature communications·2025
Same author

[Udo Martin Helmchen : 12 February 1939-18 January 2025].

Pathologie (Heidelberg, Germany)·2025

Surgical stress significantly alters gene expression in prostate cancer tissues. Preoperative tissue collection via core biopsies is optimal for accurate molecular analysis, challenging immediate postoperative sampling.

Area of Science:

  • Oncology
  • Molecular Biology
  • Surgical Pathology

Background:

  • Gene expression analysis is crucial for understanding cancer biology.
  • Perioperative and postoperative stress can rapidly alter RNA quality and transcript levels.
  • Previous studies indicate gene expression changes occur during and after organ removal.

Purpose of the Study:

  • To evaluate the impact of surgical manipulation and time on gene expression in prostate cancer.
  • To determine the optimal timing for tissue collection for molecular studies in prostate cancer.
  • To compare perioperative and postoperative gene expression profiles.

Main Methods:

  • Quantitative reverse transcription-polymerase chain reactions (RT-qPCR) were used.
  • Analysis focused on 91 cancer-related genes.

Related Experiment Videos

  • Samples were collected from normal and cancerous prostate tissues of 10 patients at eight time points during and after surgery.

    • Main Results:

    • Eight out of 91 genes (EGR1, p21, KRT17, PIM1, S100P, TNFRSF, WFDC2, and TRIM29) showed significant time-dependent changes.
    • All eight genes were upregulated, with the most prominent changes occurring intraoperatively.
    • Postoperative changes within the first hour were less significant.

    Conclusions:

    • Immediate postoperative tissue sampling may compromise the utility of gene expression analysis.
    • Preoperative tissue collection through core biopsies is recommended for studying molecular alterations in prostate tissues.
    • Findings suggest a need to reconsider tissue collection protocols in prostate cancer research.