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Related Experiment Videos

Ba(2+)-induced action potentials in osteoblastic cells.

J Ferrier1, C Grygorczyk, R Grygorczyk

  • 1Medical Research Council Group in Periodontal Physiology, University of Toronto, Ontario, Canada.

The Journal of Membrane Biology
|September 1, 1991
PubMed
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Barium ions (Ba2+) induce action potentials in rat osteosarcoma cells by affecting L-type calcium channels. This study explores the mechanism of these Ba2+-induced electrical events in bone cells.

Area of Science:

  • Cellular Electrophysiology
  • Ion Channel Physiology
  • Bone Cell Biology

Background:

  • Osteoblast-like cells (ROS 17/2.8) exhibit electrical activity.
  • Barium ions (Ba2+) are known to affect ion channels.

Purpose of the Study:

  • To investigate the induction of action potentials by Ba2+ in osteosarcoma cells.
  • To elucidate the role of L-type calcium channels in Ba2+-induced electrical activity.

Main Methods:

  • Current clamp and voltage clamp electrophysiology on rat osteosarcoma cells.
  • Microelectrode and whole-cell patch-clamp techniques were employed.
  • Pharmacological agents (BAY K 8644, nifedipine, Cd2+) and varying ion concentrations were used.

Main Results:

Related Experiment Videos

  • Ba2+ induced long-duration depolarizing pulses and inward current pulses.
  • These pulses were dependent on temperature and Ba2+ concentration.
  • The observed phenomena were mediated by L-type calcium channels and blocked by nifedipine and Cd2+.

Conclusions:

  • Ba2+ can induce action potentials in osteosarcoma cells via L-type calcium channels.
  • A model suggests voltage-independent channel opening by cytosolic Ba2+.
  • Channel inactivation and refractory periods are crucial for repeated pulsing.