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Protective immunity conferred by recombinant Pasteurella multocida lipoprotein E (PlpE).

Jin-Ru Wu¹, Jui-Hung Shien, Happy K Shieh

¹Graduate Institute of Veterinary Microbiology, National Chung Hsing University, Taichung 402, Taiwan.

|April 24, 2007

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Recombinant Pasteurella multocida lipoprotein E (PlpE) provides cross-protection in mice and chickens against lethal challenge. This study highlights PlpE as a potential broad-acting antigen for P. multocida vaccines.

Area of Science:

Veterinary Microbiology
Immunology
Molecular Biology

Background:

Pasteurella multocida is a significant bacterial pathogen causing respiratory and systemic diseases in various animal species.
Lipoproteins are key surface components of P. multocida involved in host-pathogen interactions.
Developing effective vaccines against P. multocida remains a challenge due to antigenic diversity.

Purpose of the Study:

To clone and express genes for Pasteurella multocida lipoprotein E (PlpE) and lipoprotein B (PlpB).
To evaluate the immunoprotective efficacy of recombinant PlpE (r-PlpE) and r-PlpB in animal models.
To investigate the potential of PlpE as a cross-protective antigen against different P. multocida strains.

Main Methods:

Genes for PlpE and PlpB were cloned from P. multocida strain X-73 and expressed in Escherichia coli.

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Recombinant proteins (r-PlpE and r-PlpB) were purified.

Mice and chickens were immunized with purified r-PlpE or r-PlpB.

Immunized animals were challenged with virulent P. multocida strains, and survival rates were assessed.

Sequence analysis of PlpE from different P. multocida strains was performed.

Main Results:

Mice immunized with r-PlpE showed 80-100% protection against challenge with multiple P. multocida serotypes.
Chickens immunized with r-PlpE demonstrated 63-100% protection against lethal challenge.
Immunization with r-PlpB did not confer significant protection in either mice or chickens.
Sequence analysis revealed high (90.8-100%) identity among PlpE proteins from different P. multocida strains.

Conclusions:

Recombinant PlpE elicits protective immunity and confers cross-protection against various Pasteurella multocida strains in mice and chickens.
PlpE is a promising candidate antigen for developing a broad-spectrum P. multocida vaccine.
This study reports the first recombinant P. multocida antigen demonstrating cross-protective capabilities in animals.