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Related Concept Videos

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...

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Related Experiment Video

Updated: Jul 15, 2026

Identification of Circular RNAs using RNA Sequencing
08:25

Identification of Circular RNAs using RNA Sequencing

Published on: November 14, 2019

Identification and characterization of human non-coding RNAs with tissue-specific expression.

Yasnory T F Sasaki1, Miho Sano, Takashi Ideue

  • 1Functional RNA Research Team, Biological Information Research Center, National Institute of Advanced Industrial Science and Technology (AIST), Tokyo 135-0064, Japan.

Biochemical and Biophysical Research Communications
|April 25, 2007
PubMed
Summary

This study analyzed non-coding RNAs (ncRNAs) in human tissues, finding most exhibit tissue-specific expression and low abundance. Expression patterns suggest conservation between human and mouse ncRNAs, even those without direct counterparts.

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A Rapid High-throughput Method for Mapping Ribonucleoproteins (RNPs) on Human pre-mRNA
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Published on: December 2, 2009

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Identification of Circular RNAs using RNA Sequencing
08:25

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Published on: November 14, 2019

A Rapid High-throughput Method for Mapping Ribonucleoproteins (RNPs) on Human pre-mRNA
13:00

A Rapid High-throughput Method for Mapping Ribonucleoproteins (RNPs) on Human pre-mRNA

Published on: December 2, 2009

Area of Science:

  • Genomics
  • Molecular Biology
  • Transcriptomics

Background:

  • Non-coding RNAs (ncRNAs) play crucial roles in gene regulation.
  • Understanding the expression patterns of ncRNAs is essential for elucidating their functions.
  • The H-Invitational database provides a valuable resource for identifying and studying ncRNAs.

Purpose of the Study:

  • To investigate the expression profiles of selected ncRNAs across 11 human tissues.
  • To determine the abundance and tissue specificity of these ncRNAs.
  • To explore the evolutionary conservation of ncRNA expression between humans and mice.

Main Methods:

  • Selection of 150 ncRNA clones from the H-Invitational database based on gene structure and EST information.
  • Expression profiling using quantitative RT-PCR and Northern blot hybridization.
  • Absolute quantification of representative ncRNAs.
  • Comparative genomic analysis to identify mouse counterparts.

Main Results:

  • 67% of selected ncRNAs displayed tissue-specific expression patterns.
  • The majority of ncRNAs were found to be low abundance transcripts.
  • Only 27% of the studied human ncRNAs had identifiable mouse counterparts.
  • Expression patterns of human ncRNAs without mouse counterparts were similar to those of mouse ncRNAs.

Conclusions:

  • Most analyzed ncRNAs are tissue-specific and expressed at low levels.
  • ncRNA expression patterns appear to be conserved between humans and mice, suggesting functional importance.
  • Further research is needed to fully understand the roles of ncRNAs with limited cross-species conservation.