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Updated: Jul 15, 2026

Chromosome Preparation From Cultured Cells
Published on: January 28, 2014
Isodicentric Philadelphia chromosomes in imatinib mesylate (Gleevec)-resistant patients
Christine M Szych1, Jane L Liesveld, M Anwar Iqbal
1Department of Pathology, University of Rochester, Strong Memorial Hospital, 601 Elmwood Avenue, P.O. Box 704, Rochester, NY 14642, USA.
Abstract:
Amplification/duplication of the BCR-ABL gene has been found to be one of the key factors leading to drug resistance to imatinib mesylate (IM). In the present study, we used G-banding to identify the presence of de novo identical isodicentric chromosomes in IM-resistant patients. Fluorescence in situ hybridization (FISH) analysis on interphase nuclei confirmed the heterogeneity and amplification of the fused BCR-ABL gene. FISH analysis superimposed on G-banding confirmed the presence of isodicentric Philadelphia chromosomes. The impact of the isodicentric Philadelphia chromosomes on genomic instability, heterogeneity, and amplification of the Philadelphia chromosomes in IM-resistant patients is discussed.
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