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Potential interface between ribosomal protein production and pre-rRNA processing.

Dipayan Rudra1, Jaideep Mallick, Yu Zhao

  • 1Department of Cell Biology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.

Molecular and Cellular Biology
|April 25, 2007
PubMed
Summary
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The CURI complex, containing Ifh1, regulates ribosomal protein gene transcription in yeast. Its interaction with pre-ribosomal RNA processing factors links transcription and ribosome synthesis.

Area of Science:

  • Molecular Biology
  • Yeast Genetics
  • Gene Regulation

Background:

  • Ribosomal protein gene transcription in Saccharomyces cerevisiae is crucial for ribosome biogenesis.
  • Transcription is controlled by Rap1, Fhl1, and Ifh1, with Ifh1's presence correlating with active transcription.

Purpose of the Study:

  • To investigate the interaction network of transcription factors controlling ribosomal protein gene expression.
  • To elucidate the role of Ifh1 in coordinating transcription with pre-ribosomal RNA processing.

Main Methods:

  • Co-immunoprecipitation to identify protein complexes.
  • In vitro kinase assays to determine phosphorylation activity.
  • Gene depletion studies to assess protein function in vivo.

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Main Results:

  • Ifh1 forms the CURI complex with casein kinase 2 (CK2), Utp22, and Rrp7.
  • CK2 within the CURI complex phosphorylates Ifh1.
  • Depletion of Utp22 or Rrp7, essential for pre-rRNA processing, increases ribosomal protein mRNA levels.

Conclusions:

  • The CURI complex acts as a crucial mediator between pre-ribosomal RNA processing and ribosomal protein gene transcription.
  • This links the two parallel pathways essential for yeast ribosome synthesis.