Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Growth of Cartilage and Bone Tissue

Growth of Cartilage and Bone Tissue

Chondrocytes form a temporary cartilaginous model by dividing and secreting a thick gel-like extracellular matrix. Once the chondrocytes undergo programmed cell death, osteoblasts enter the site of the cartilaginous model. The process of replacing the temporary cartilaginous model with bone in an ordered manner is called endochondral ossification. In endochondral ossification, not all of the cartilage is replaced by bone tissue. Some cartilage that performs a protective and supportive function...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

NiO@rGO nanohybrids triggered cytotoxicity via oxidative stress, cell cycle arrest, and apoptosis pathways.

Scientific reports·2025

Same author

Retraction Note: TiO2 nanoparticles potentiated the cytotoxicity, oxidative stress and apoptosis response of cadmium in two different human cells.

Environmental science and pollution research international·2025

Same author

Toxic Effects of Synthesized Bismuth Oxide/Reduced Graphene Oxide (Bi<sub>2</sub>O<sub>3</sub>/RGO) Nanocomposites in Two Distinct Mammalian Cell Lines: Role Oxidative Stress and Apoptosis.

International journal of nanomedicine·2024

Same author

Chemical synthesis, characterization, and anticancer potential of CuO/ZrO<sub>2</sub>/TiO<sub>2</sub>/RGO nanocomposites against human breast (MCF-7) cancer cells.

RSC advances·2024

Same author

Green hydrothermal synthesis and characterization of Ag<sub>2</sub>O-supported MgO/rGO nanocomposites by using Phoenix leaf extract: a promising approach for enhanced photocatalytic and anticancer activities.

Environmental science and pollution research international·2024

Same author

Green synthesis of ZnO-TiO<sub>2</sub>/RGO nanocomposites using <i>Senna surattensis</i> extract: a novel approach for enhanced anticancer efficacy and biocompatibility.

RSC advances·2024

Search research articles

Related Experiment Video

Updated: Jul 15, 2026

Addressing Practical Issues in Atomic Force Microscopy-Based Micro-Indentation on Human Articular Cartilage Explants

Addressing Practical Issues in Atomic Force Microscopy-Based Micro-Indentation on Human Articular Cartilage Explants

Published on: October 28, 2022

Morphological changes in articular cartilage due to static compression: polarized light microscopy study.

Hisham A Alhadlaq¹, Yang Xia, Fay M Hansen

¹Department of Physics and Center for Biomedical Research, Oakland University, Rochester, Michigan 48309, USA.

Connective Tissue Research

|April 25, 2007

Summary

A new histology method effectively studied cartilage deformation. Significant changes in collagen matrix architecture occurred above 15% strain, with fibrils aligning parallel to the surface and a new transitional zone forming.

More Related Videos

Real-time Visualization and Analysis of Chondrocyte Injury Due to Mechanical Loading in Fully Intact Murine Cartilage Explants

Real-time Visualization and Analysis of Chondrocyte Injury Due to Mechanical Loading in Fully Intact Murine Cartilage Explants

Published on: January 7, 2019

Related Experiment Videos

Last Updated: Jul 15, 2026

Addressing Practical Issues in Atomic Force Microscopy-Based Micro-Indentation on Human Articular Cartilage Explants

Addressing Practical Issues in Atomic Force Microscopy-Based Micro-Indentation on Human Articular Cartilage Explants

Published on: October 28, 2022

Real-time Visualization and Analysis of Chondrocyte Injury Due to Mechanical Loading in Fully Intact Murine Cartilage Explants

Real-time Visualization and Analysis of Chondrocyte Injury Due to Mechanical Loading in Fully Intact Murine Cartilage Explants

Published on: January 7, 2019

Area of Science:

Biomedical Engineering
Histology
Orthopedics

Background:

Articular cartilage extracellular matrix (ECM) deformation is crucial for joint function.
Understanding ECM changes under compression is vital for diagnosing and treating cartilage pathologies.
Existing histological methods may not accurately preserve the native architecture of compressed cartilage.

Purpose of the Study:

To develop and validate a novel compression-preservation histology method for studying articular cartilage ECM deformation.
To investigate the effects of varying strain levels on collagen fibril orientation and organization in canine cartilage.

Main Methods:

A Hoffman clamp was used to compress canine cartilage-bone blocks (n=14) at strain levels from 5% to 65%.
The compression was maintained throughout the paraffin embedding and microtoming process.
Histological sections were analyzed using polarized light microscopy to map fibril orientation (angle) and organization (retardance).

Main Results:

Little change in angle and retardance profiles was observed for strain levels between 0% and 15%.
Significant alterations in these profiles occurred at strain levels of 15% and above.
Higher compression led to increased alignment of collagen fibrils parallel to the articular surface.
A distinct "transitional zone" formed in the deep tissue layer at approximately 30% strain.

Conclusions:

The novel compression-preservation method effectively preserves and reveals altered collagen matrix architecture in compressed cartilage.
This technique provides valuable insights into the mechanical behavior and structural remodeling of articular cartilage ECM.
Findings highlight critical strain thresholds impacting cartilage structural integrity and organization.