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Improved Enzyme Protection Assay to Study Staphylococcus aureus Internalization and Intracellular Efficacy of Antimicrobial Compounds
Published on: September 8, 2021
In vitro antimicrobial effect of encapsulated vancomycin on internalized Staphylococcus aureus within endothelial
Henry Nettey1, Dinesh Haswani, Martin D'Souza
1Department of Pharmaceutical Sciences, Southern School of Pharmacy, Mercer University, Atlanta, GA, USA. hnettey@msn.com
Abstract:
Vancomycin (VCN) is a glycopeptide antibiotic that is effective in the treatment of gram-positive bacterial infections, but mainly reserved for methicilin resistant Staphylococcus aureus. It is, however, ineffective against intracellular bacteria and hence a particulate form of VCN would be required. Bovine serum albumin (BSA) microspheres of VCN with a mean particle size of 5 +/- 1.6 microm were used. Human microvascular endothelial cells internalized both S. aureus and VCN microspheres in a time and concentration-dependent manner, however, the uptake was inhibited by cytochalasin D. Action of VCN on S. aureus in the intracellular microenvironment decreased the bacterial load considerably.
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