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Related Experiment Video

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An Automated Culture System for Use in Preclinical Testing of Host-Directed Therapies for Tuberculosis

An Automated Culture System for Use in Preclinical Testing of Host-Directed Therapies for Tuberculosis

Published on: August 16, 2021

A study of phage based diagnostic technique for tuberculosis.

Purabi Barman¹, Deepa Gadre

¹Department of Microbiology, University College of Medical Sciences and Guru Teg Bahadur Hospital, Delhi- 10095. purabiduorah@sify.com

The Indian Journal of Tuberculosis

|April 26, 2007

Summary

The Fast Plaque TB assay offers a rapid and reliable method for diagnosing tuberculosis (TB). This phage assay demonstrates high sensitivity and specificity, outperforming conventional diagnostic tools for Mycobacterium tuberculosis detection.

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Published on: January 17, 2014

Area of Science:

Microbiology
Infectious Diseases
Diagnostic Technology

Background:

Tuberculosis (TB) is a major infectious disease in India, with diagnosis challenged by slow Mycobacterium tuberculosis growth.
Conventional TB diagnostic tools require significant time due to the slow growth rate of Mycobacterium tuberculosis.

Purpose of the Study:

To compare the diagnostic efficacy of the novel Fast Plaque TB assay against conventional methods for tuberculosis diagnosis.
To evaluate the sensitivity and specificity of the phage assay for detecting Mycobacterium tuberculosis in clinical samples.

Main Methods:

Two hundred and twelve clinically suspected TB cases were enrolled.
Three consecutive sputum samples were collected for Ziehl-Neelsen (ZN) staining, culture on LJ media, and phage assay.
Phage assay was performed to detect Mycobacterium tuberculosis.

Main Results:

The phage assay showed a sensitivity of 94.34% and specificity of 93.88% compared to AFB smear.
Compared to LJ media culture, the phage assay demonstrated 92.86% sensitivity and 97.83% specificity.
All 8 Non-Tubercular Mycobacteria tested were negative by the Fast Plaque Assay.

Conclusions:

The Fast Plaque TB assay is a rapid, reliable, and cost-effective method for diagnosing pulmonary tuberculosis.
The phage assay provides a significant improvement over conventional diagnostic methods for TB.