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Real-time Quaking-induced Conversion Assay for Detection of CWD Prions in Fecal Material
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Reference materials for the evaluation of pre-mortem variant Creutzfeldt-Jakob disease diagnostic assays
J K Cooper1, K Ladhani, P D Minor
1CJD Resource Centre, National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Potters Bar EN6 3QG, UK. jcooper@nibsc.ac.uk
Background And Objectives:
A standard panel of materials is needed for the evaluation of assays being developed for the diagnosis of variant Creutzfeldt-Jakob disease.
Materials And Methods:
Tissues from human and animals incubating transmissible spongiform encephalopathy disease have been prepared, aliquoted and where possible characterized by in vitro methods.
Results:
A standardized preparation of materials has been generated.
Conclusions:
Large-scale preparations of tissues and blood fractions can be used to directly compare the sensitivities of assays using different formats.
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