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[Association of CD14 gene polymorphism with atopic diseases in Chinese Han ethnic group children]
Hai-lin Zhang1, Li-yan Ni, Qi-yu Bao
1Department of Respiratory Diseases, The Children's Hospital Affiliated to Zhejiang University School of Medicine, Hangzhou 310003, China.
Insights
Single nucleotide polymorphisms (SNPs) in the CD14 gene were studied in Chinese children. The CD14/-159 SNP was present, but not associated with atopic diseases or IgE levels.
Area of Science:
- Genetics
- Immunology
- Pediatrics
Background:
- Atopic diseases are common in children.
- The CD14 gene plays a role in immune responses.
- Understanding genetic variations like single nucleotide polymorphisms (SNPs) is crucial for disease association studies.
Purpose of the Study:
- To investigate the distribution of CD14 gene SNPs in Chinese Han children from Wenzhou.
- To determine the association between CD14 gene SNPs and atopic diseases (asthma, allergic rhinitis, atopic dermatitis).
- To explore the relationship between CD14 gene SNPs and serum total IgE levels.
Main Methods:
- Sequencing of the CD14 gene in 113 children with atopic diseases and 67 healthy controls.
- Identification and characterization of single nucleotide polymorphisms (SNPs).
- Measurement of total and specific IgE levels using immunoassay and uniCAP systems.
Main Results:
- The CD14/-159 SNP was identified in the Wenzhou Han Chinese population.
- The TT genotype was the most frequent (57.0%) in healthy children and (46.9%) in atopic children.
- No significant differences were found in CD14/-159 SNP distribution or IgE levels between atopic and healthy children.
Conclusions:
- The CD14/-159 SNP is present in Wenzhou Han Chinese children.
- No association was found between the CD14/-159 SNP and atopic diseases or serum total IgE levels in this population.
- Further research may be needed to explore other genetic factors in atopic diseases.
Objective:
To investigate the distribution characteristics of the single nucleotide polymorphisms (SNPs) of the human CD14 gene in Chinese Han ethnic group children in Wenzhou, and their association with atopic diseases.
Methods:
Totally 113 cases were recruited in atopic disease group who met the following criteria: 2 - 12 years old, clinically diagnosed as asthma or allergic rhinitis or atopic dermatitis, elevation of serum total IgE levels and serum specific IgE. Sixty-seven healthy children were enrolled in control group. The related regions of CD14 gene were sequenced to identify and characterize the SNPs, and plasma TIgE and SIgE were detected by immunoassay system and uniCAP system, respectively. The frequency of genotypes and alleles between two groups, as well as the levels of IgE in different genotypes, were compared.
Results:
CD14/-159 SNP was present in Han ethnic group population of Wenzhou. The frequency of each genotype was 57.0% (TT), 28.0% (TC), 15.0% (CC) in normal children, and 46.9% (TT), 35.4% (TC), 17.7% (CC) in atopic children. No significant difference was found in the distribution of CD14/-159 polymorphism between atopic children and healthy control (chi(2) = 1.918, P > 0.05) according to Hardy-Weinberg principle statistics. There were no significant difference in frequency of each genotype between boys and girls. No significant difference was found in the total plasma IgE levels among groups of TT genotypes [(2520 +/- 460) IU/L], TC genotypes [(2400 +/- 460) IU/L] and CC genotype [(2500 +/- 460) IU/L] (F = 0.807, P > 0.05).
Conclusion:
CD14/-159 SNP is present in Han ethnic group children in Wenzhou, and other SNP in CD14 gene was not found. TT genotype was the primary genotype in CD14/-159 SNP in the children studied. No relationship between CD14/-159 SNP and atopic disease or serum total IgE level was found.
