[DNA damages in individual mammalian cells]

The method of alkaline microelectrophoresis of nucleoid DNA of isolated cells built in an agar gel was used to study the harmful effect of radiation on the genome of mouse peripheral blood lymphocytes. The yield of the lymphocyte DNA structure damages in irradiated animals and in the in vitro exposed lymphocytes was a linear function of radiation dose within the dose range from 0.5 to 5 Gy and 0.27 to 1 Gy respectively. A study was made of the dynamics of DNA structure damages in cells NIH 3T3 exposed to 3 Gy radiation, and in mouse lymphocytes exposed to 4 Gy radiation.