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Updated: Jul 15, 2026

Defining Substrate Specificities for Lipase and Phospholipase Candidates
Published on: November 23, 2016
Production and regulation of lipase activity from Penicillium restrictum in submerged and solid-state fermentations
Luciana A I de Azeredo1, Patrícia M Gomes, Geraldo L Sant'Anna
1Biochemistry Department, Institute of Chemistry, Federal University of Rio de Janeiro, Centro de Tecnologia, Bloco A, Lab. 549-2, 21945-900 Rio de Janeiro, RJ, Brazil.
Abstract:
Different carbon (C) sources, mainly carbohydrates and lipids, have been screened for their capacity to support growth and lipase production by Penicillium restrictum in submerged fermentation (SmF) and in solid-state fermentation (SSF). Completely different physiological behaviors were observed after the addition of easily (oleic acid and glucose) and complex (olive oil and starch) assimilable C sources to the liquid and solid media. Maximal lipolytic activities (12.1 U/mL and 17.4 U/g) by P. restrictum were obtained with olive oil in SmF and in SSF, respectively. Biomass levels in SmF (12.2-14.1 mg/mL) and SSF (7.0-8.0 mg/g) did not varied greatly with the distinct C sources used. High lipase production (12.3 U/g) using glucose was only attained in SSF, perhaps due to the ability of this fermentation process to minimize catabolite repression.
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