Simple and simultaneous determination for 12 phenothiazines in human serum by reversed-phase high-performance liquid...

Area of Science:

• Analytical Chemistry
• Pharmacology

Background:

• Phenothiazines are a class of drugs with various therapeutic applications.
• Accurate quantification of phenothiazines in biological samples is crucial for clinical monitoring and forensic toxicology.

Purpose of the Study:

• To develop and validate a high-performance liquid chromatographic (HPLC) method for the simultaneous determination of 12 phenothiazines in human serum.
• To establish a sensitive, selective, and reliable analytical method for clinical and forensic analysis of phenothiazines.

Main Methods:

• Simultaneous analysis of 12 phenothiazines using a C(18) reversed-phase column and HPLC with UV detection.
• Optimization of mobile phase composition (acetonitrile-methanol-30 mM NaH(2)PO(4), pH 5.6) and flow rate (0.9 mL/min).
• UV detection at 250 nm.

Main Results:

• High recoveries (87.6-99.8%) for the 12 phenothiazines spiked into serum samples.
• Excellent linearity (regression equations) with low detection limits (3.2-5.5 ng/mL).
• Low inter-day and intra-day coefficients of variation (commonly below 8.8%) indicating good precision and accuracy.

Conclusions:

• The developed HPLC/UV method is selective, accurate, and precise for simultaneous phenothiazine analysis in human serum.
• This method is suitable for both clinical and forensic purposes, enabling the screening and quantification of commonly available phenothiazines.
• The method provides a valuable tool for therapeutic drug monitoring and toxicological investigations involving phenothiazines.