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Updated: Jul 15, 2026

Peptide:MHC Tetramer-based Enrichment of Epitope-specific T cells
Published on: October 22, 2012
The final touches make perfect the peptide-MHC class I repertoire
Gianna Elena Hammer1, Takayuki Kanaseki, Nilabh Shastri
1Division of Immunology, Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720-3200, USA.
Abstract:
Major histocompatibility complex (MHC) class I molecules present short, perfectly cleaved peptides on the cell surface for immune surveillance by CD8(+) T cells. The pathway for generating these peptides begins in the cytoplasm, and the peptide-MHC I (pMHC I) repertoire is finalized in the endoplasmic reticulum. Recent studies show that the peptides for MHC I are customized by the ER aminopeptidase associated with antigen processing and by dynamic interactions within the MHC peptide-loading complex. Failure to customize the pMHC I repertoire has profound immunological consequences.
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