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Updated: Jul 15, 2026

High-resolution Imaging and Analysis of Individual Astral Microtubule Dynamics in Budding Yeast
Published on: April 20, 2017
Protein arms in the kinetochore-microtubule interface of the yeast DASH complex
J J L Miranda1, David S King, Stephen C Harrison
1Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA 02138, USA.
Abstract:
The yeast DASH complex is a heterodecameric component of the kinetochore necessary for accurate chromosome segregation. DASH forms closed rings around microtubules with a large gap between the DASH ring and the microtubule cylinder. We characterized the microtubule-binding properties of limited proteolysis products and subcomplexes of DASH, thus identifying candidate polypeptide extensions involved in establishing the DASH-microtubule interface. The acidic C-terminal extensions of tubulin subunits are not essential for DASH binding. We also measured the molecular mass of DASH rings on microtubules with scanning transmission electron microscopy and found that approximately 25 DASH heterodecamers assemble to form each ring. Dynamic association and relocation of multiple flexible appendages of DASH may allow the kinetochore to translate along the microtubule surface.
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