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Related Experiment Video

Updated: Jul 15, 2026

Ratiometric Biosensors that Measure Mitochondrial Redox State and ATP in Living Yeast Cells

Ratiometric Biosensors that Measure Mitochondrial Redox State and ATP in Living Yeast Cells

Published on: July 22, 2013

Proteome alterations in rat mitochondria caused by aging.

Norbert A Dencher¹, Monika Frenzel, Nicole H Reifschneider

¹Physical Biochemistry, Department of Chemistry, Darmstadt University of Technology, Petersenstr., 22, D-64287 Darmstadt, Germany. nad@pop.tu-darmstadt.de

Annals of the New York Academy of Sciences

|April 27, 2007

Summary

Mitochondrial protein changes during aging, particularly in the inner membrane, offer insights into aging and related diseases. Alterations in ATP synthase and OXPHOS supercomplexes in rat brains link respiration to longevity and ROS production.

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Area of Science:

Mitochondrial biology
Aging research
Proteomics

Background:

Mitochondrial protein analysis is key to understanding aging and age-related diseases.
The inner mitochondrial membrane proteome, including respiratory chain complexes, is crucial for life-span control.
Age-dependent variations in mitochondrial proteins are examined in various rat tissues.

Purpose of the Study:

To investigate age-dependent variations in the mammalian mitochondrial membrane proteome.
To explore the role of mitochondrial proteins and their supramolecular organization in aging.
To identify age-related changes in protein abundance, composition, structure, and activity.

Main Methods:

Native polyacrylamide gel electrophoresis (native PAGE) to separate proteins and supercomplexes in their native state.
MALDI-TOF mass spectrometry for protein identification and quantification.
Analysis of protein variations in rat brain cortex during aging.

Main Results:

Detected age-modulated differences in mitochondrial and non-mitochondrial proteins (e.g., Na,K-ATPase, HSP60, aconitase-2, V-type ATPase, ATP synthase, OXPHOS complexes I-IV) in rat brain.
Observed a decrease in intact MF(o)F(1) ATP synthase during aging in the cortex.
Found age-related alterations in the oligomerization of MF(o)F(1) ATP synthase and the abundance of OXPHOS supercomplexes.

Conclusions:

Age-related changes in MF(o)F(1) ATP synthase oligomerization may link respiration to longevity.
Alterations in the supramolecular architecture of OXPHOS complexes could explain age-related changes in ROS production.
Mitochondrial proteome analysis provides insights into aging mechanisms and potential therapeutic targets.