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Related Experiment Video

Updated: Jul 15, 2026

Preparation and Culture of Myogenic Precursor Cells/Primary Myoblasts from Skeletal Muscle of Adult and Aged Humans

Preparation and Culture of Myogenic Precursor Cells/Primary Myoblasts from Skeletal Muscle of Adult and Aged Humans

Published on: February 16, 2017

Age-dependent effects on functional aspects in human satellite cells.

S Beccafico¹, C Puglielli, T Pietrangelo

¹Department of Basic and Applied Medical Sciences, University "G. d'Annunzio" Nuovo Polo Didattico Pal. B, Via dei Vestini 29, Chieti I-66013, Italy.

Annals of the New York Academy of Sciences

|April 27, 2007

Summary

Aging causes oxidative damage and sarcopenia, a loss of muscle mass and strength. This study reveals age-dependent calcium handling defects in muscle satellite cells, impacting their repair function.

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Isolation, Culture, Characterization, and Differentiation of Human Muscle Progenitor Cells from the Skeletal Muscle Biopsy Procedure

Isolation, Culture, Characterization, and Differentiation of Human Muscle Progenitor Cells from the Skeletal Muscle Biopsy Procedure

Published on: August 23, 2019

Area of Science:

Muscle physiology
Cellular aging
Sarcopenia research

Background:

Aging leads to oxidative damage and muscle functional decline (sarcopenia).
Skeletal muscle satellite cells, crucial for repair, may also age and exhibit altered function.
Calcium (Ca2+) homeostasis is vital for muscle function and satellite cell activity.

Purpose of the Study:

To investigate age-dependent changes in skeletal muscle satellite cells.
To examine the impact of aging on Ca2+ handling and excitation-contraction (EC) coupling in myotubes derived from aged muscle.

Main Methods:

Cultured myotubes derived from aged and young muscle donors.
In vitro differentiation of satellite cells into myotubes.
Single-cell Ca2+ imaging using various stimuli (ATP, caffeine, KCl).
Assessment of lipid peroxidation as a marker of oxidative damage.

Main Results:

Increased lipid peroxidation in aged myotubes.
Age-dependent alterations in Ca2+ transient production in response to stimuli.
Evidence of defective EC coupling in aged myotubes, suggesting impaired RyR1 and DHPR interaction.
Ca2+ release via RyR1 is dependent on extracellular Ca2+ after sarcolemmal depolarization in aged myotubes.

Conclusions:

Skeletal muscle satellite cells undergo aging, mirroring changes in mature muscle fibers.
Age-related oxidative stress contributes to impaired Ca2+ homeostasis and EC coupling in satellite cells.
Defective satellite cell function may hinder muscle repair processes in aged individuals.