Related Experiment Video
Updated: Jul 15, 2026

Probiotic Studies in Neonatal Mice Using Gavage
Published on: January 27, 2019
Impact of diet on the intestinal microbiota in 10-month-old infants
Susanne Nielsen1, Dennis S Nielsen, Lotte Lauritzen
1Department of Human Nutrition, Centre of Advanced Food Studies, Faculty of Life Sciences, University of Copenhagen, Frederiksberg, Denmark.
Objective:
To investigate whether diet influences the composition of the intestinal microbiota in 10-month-old infants.
Patients And Methods:
Fecal samples were collected from sixty-five 10-month-old infants participating in a randomized 2 x 2 intervention study comparing cow's milk (CM) with infant formula (IF) with or without fish oil (FO) supplement. Infants randomized to CM received a daily iron supplement. Bacterial DNA was extracted from the feces. Polymerase chain reaction was performed with primers targeting the V3 and V6-8 region of the 16S rRNA gene and analyzed by denaturing gradient gel electrophoresis (DGGE). Cluster analysis of the DGGE gels was performed by use of the Pearson correlation coefficient.
Results:
Samples from infants receiving CM clustered differently than did those from the IF group in the V3-based DGGE gels (P < 0.001) and showed a different distribution with or without FO in the CM group (P = 0.001) but not in the IF group (P = 0.39). Repeat analysis with the V6-8-based DGGE gels showed the same pattern, although the V3 gels had 2.5 times as many bands as the V6-8 gels.
Conclusions:
Consumption of CM or IF has a decisive influence on the composition of the intestinal microbiota. Supplementation with FO showed an effect on the microbiota only in the CM group. We speculate that these differences could be influenced by the intake of iron and n-3 polyunsaturated fatty acids, respectively.
More Related Videos
07:15An In Vitro Batch-culture Model to Estimate the Effects of Interventional Regimens on Human Fecal Microbiota
Published on: July 31, 2019
09:36Effect of Hyaluronic Acid 35 kDa on an In Vitro Model of Preterm Small Intestinal Injury and Healing Using Enteroid-Derived Monolayers
Published on: July 28, 2022
Related Concept Videos
Anatomy of the Intestines
Small Intestines
The small intestine is an ~7 meter-long tube with an inner diameter of just 2.5 cm. Since most nutrients are absorbed here, the inner lining of the small...
Development of Human Microbiota
Microbiota Modulation by Antibiotics
Development of the Oral Microbiota
Microbiota of the Stomach and Small Intestine
Dysbiosis of the Gut Microbiota