Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Visualizing RNA splicing in vivo.

Gayatri Gowrishankar1, Jianghong Rao

  • 1Molecular Imaging Program at Stanford, Department of Radiology & Bio-X Program, Cancer Biology Program, Stanford University School of Medicine, 1201 Welch Road, Stanford, California 94305-5484, USA. jrao@stanford.edu

Molecular Biosystems
|April 27, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Imaging of <i>Staphylococcus aureus</i> infections and biofilms using a selective covalent probe for the unique serine hydrolase FphE.

bioRxiv : the preprint server for biology·2026
Same author

Imaging of Staphylococcus aureus Infections and Biofilms Using a Selective Covalent Probe for the Unique Serine Hydrolase FphE.

Angewandte Chemie (International ed. in English)·2026
Same author

Mycobacteriophage Functionalized Magnetic Nanocrystal Clusters for Highly Sensitive and Rapid Detection of <i>Mycobacterium tuberculosis</i>.

JACS Au·2025
Same author

B7-H4 ImmunoPET Imaging Tracks Tumor-Associated Macrophage Changes in Prostate Cancer.

Molecular pharmaceutics·2025
Same author

Augmentation of [<sup>18</sup>F]-C-SNAT4 PET imaging of apoptosis after radiotherapy using a cold mixing strategy.

EJNMMI research·2025
Same author

Copper Chelation Induces Morphology Change in Mitochondria of Triple-Negative Breast Cancer.

JACS Au·2025

Researchers are visualizing RNA splicing in living cells and animals using Tetrahymena ribozymes. This advancement holds potential for gene therapy applications, enabling real-time monitoring of RNA splicing processes.

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Gene Therapy

Background:

  • Ribozymes are RNA molecules with catalytic activity, notably RNA splicing.
  • The Tetrahymena thermophila ribozyme is key for eukaryotic RNA splicing.
  • This ribozyme shows promise as a gene therapy agent.

Purpose of the Study:

  • To review progress in visualizing RNA splicing in living mammalian cells.
  • To highlight advancements in imaging RNA splicing in living animals.
  • To explore the potential of ribozyme-mediated RNA splicing for gene therapy.

Main Methods:

  • Utilizing the beta-lactamase reporter system for visualizing RNA splicing in single living mammalian cells.
  • Employing the luciferase reporter system for imaging RNA splicing in living animals.

Related Experiment Videos

Main Results:

  • Demonstrated visualization of Tetrahymena ribozyme-mediated RNA splicing in living cells.
  • Developed methods for imaging RNA splicing in vivo using reporter systems.

Conclusions:

  • Significant progress has been made in visualizing RNA splicing in real-time within living systems.
  • Reporter systems offer powerful tools for studying ribozyme function and gene therapy applications.
  • These advancements pave the way for improved gene therapy strategies and molecular diagnostics.