Determination of trans-resveratrol in wine by micro-HPLC with fluorescence detection

A new method for the quantitative determination of trans-resveratrol in wine has been optimized and validated. The method is based on the direct injection of 500 nL of wine in an HPLC system fitted with an RP microcolumn of 10 cm x 0.32 mm and spectrofluorometric detection. The linear dynamic range of the method covers the normal range of occurrence of the analyte in wines and extends for two orders of magnitude with r(2)= 0.9994. Twenty-three wines have been analyzed with the proposed method, finding concentrations in the range reported by other authors.