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Updated: Jul 15, 2026

Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns
Published on: April 26, 2016
Coupled-column liquid chromatography combined with postcolumn photochemical derivatization and fluorescence detection
Ahmed R Mughari1, María Martínez Galera, Piedad Parrilla Vázquez
1Department of Analytical Chemistry, University of Almeria, La Cañada de San Urbano, Almería, Spain.
Abstract:
This study examines the application of coupled-column LC-photochemically induced fluorimetry-fluorescence detection (LC-LC-PIF-FD), demonstrating its potential for the quantitative and selective detection of six herbicides, including propanil and the phenylureas monuron, monolinuron, chlorotoluron, diuron and neburon in groundwater samples. An AQUASIL C18 50 x 4.6 mm(2) id column coupled to an AQUASIL C18 150 x 4.6 mm(2) id column for analyte clean-up and determination were used, respectively. A simple SPE with Cl8 cartridges was carried out, yielding average recoveries between 80 and 112% (n = 6) with RSDs between 0.5 and 9%. The LODs ranged from 0.0083 to 0.0833 microg/L in the groundwater samples.
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