Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

Three-Dimensional Microscopy in Microbiology

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...

Overview of Microscopy Techniques

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Characterising interleukin-27 (IL-27) responses in human blood derived macrophage cells.

Cytokine·2025

Same author

Tumour purity assessment with deep learning in colorectal cancer and impact on molecular analysis.

The Journal of pathology·2024

Same author

Subcellular Epithelial HMGB1 Expression Is Associated with Colorectal Neoplastic Progression, Male Sex, Mismatch Repair Protein Expression, Lymph Node Positivity, and an 'Immune Cold' Phenotype Associated with Poor Survival.

Cancers·2023

Same author

Interleukin-27 Regulates the Function of the Gastrointestinal Epithelial Barrier in a Human Tissue-Derived Organoid Model.

Biology·2022

Same author

Gut Mucosal Microbiome Signatures of Colorectal Cancer Differ According to BMI Status.

Frontiers in medicine·2022

Same author

Identification of a prognostic signature in colorectal cancer using combinatorial algorithm-driven analysis.

The journal of pathology. Clinical research·2022

Search research articles

Related Experiment Video

Updated: Jul 15, 2026

Laser Microdissection for Species-Agnostic Single-Tissue Applications

Laser Microdissection for Species-Agnostic Single-Tissue Applications

Published on: March 31, 2022

An overview of laser microdissection technologies.

Graeme I Murray¹

¹Department of Pathology, University of Aberdeen, Foresterhill, Aberdeen AB25 2ZD, Scotland, UK. g.i.murray@abdn.ac.uk

Acta Histochemica

|April 28, 2007

Summary

Laser microdissection enables precise cell isolation for molecular analysis. Techniques like laser capture and cutting microdissection offer powerful tools for cell biology and pathology research.

Area of Science:

Biotechnology
Molecular Biology
Histology

Background:

Laser-based systems facilitate the isolation of specific cell types for molecular analysis.
Two primary laser microdissection technologies exist: laser capture microdissection and laser cutting microdissection.

Purpose of the Study:

To outline the principles, advantages, and drawbacks of laser capture and laser cutting microdissection.
To discuss critical cell and tissue preparation methods for successful microdissection and molecular studies.

Main Methods:

Review of laser capture microdissection principles.
Review of laser cutting microdissection principles.
Discussion of cell and tissue preparation techniques, including fixation and staining.

More Related Videos

Laser Capture Microdissection of Mammalian Tissue

Laser Capture Microdissection of Mammalian Tissue

Published on: October 1, 2007

Industrialized, Artificial Intelligence-guided Laser Microdissection for Microscaled Proteomic Analysis of the Tumor Microenvironment

Industrialized, Artificial Intelligence-guided Laser Microdissection for Microscaled Proteomic Analysis of the Tumor Microenvironment

Published on: June 3, 2022

Related Experiment Videos

Last Updated: Jul 15, 2026

Laser Microdissection for Species-Agnostic Single-Tissue Applications

Laser Microdissection for Species-Agnostic Single-Tissue Applications

Published on: March 31, 2022

Laser Capture Microdissection of Mammalian Tissue

Laser Capture Microdissection of Mammalian Tissue

Published on: October 1, 2007

Industrialized, Artificial Intelligence-guided Laser Microdissection for Microscaled Proteomic Analysis of the Tumor Microenvironment

Industrialized, Artificial Intelligence-guided Laser Microdissection for Microscaled Proteomic Analysis of the Tumor Microenvironment

Published on: June 3, 2022

Main Results:

Laser capture microdissection utilizes distinct principles for cell isolation.
Laser cutting microdissection offers an alternative approach to cell isolation.
Proper sample preparation is vital for effective laser microdissection and subsequent molecular analyses.

Conclusions:

Laser microdissection techniques integrate morphological and molecular analyses for advanced research.
These techniques provide significant insights into cell biology and pathology.
Appropriate application of laser microdissection enhances molecular studies.