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Vitreous domains in an aqueous ribose solution.

J M Wasylyk¹, J G Baust

¹Center for Cryobiological Research, State University of New York, Binghamton 13901.

Advances in Experimental Medicine and Biology

|January 1, 1991

Summary

Aqueous d-ribose solutions exhibit multiple vitreous domains at low temperatures, indicated by two distinct glass transitions. Water crystallization occurs at -18°C, revealing complex thermal behavior in these solutions.

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Area of Science:

Physical Chemistry
Biochemistry
Materials Science

Background:

Aqueous solutions, particularly those containing sugars like d-ribose, are crucial in various biological and industrial processes.
Understanding the phase behavior of these solutions at low temperatures is essential for applications such as cryopreservation and food science.
Previous studies have explored the glass transition of various aqueous sugar solutions, but the presence of multiple vitreous domains requires further investigation.

Purpose of the Study:

To investigate the low-temperature phase behavior of aqueous d-ribose solutions.
To identify and characterize the presence of multiple vitreous domains using thermal analysis.
To determine the crystallization temperature of bulk water within the d-ribose solution.

Main Methods:

Differential scanning calorimetry (DSC) was employed to analyze the thermal events during cooling and warming cycles.
The cooling and warming rates were maintained at a constant 5°C·min⁻¹.
Heat capacity, enthalpy changes, and their derivatives were calculated for each observed thermal event.
Relaxation studies were performed to further characterize the observed glass transitions.

Main Results:

Aqueous d-ribose solutions exhibited two distinct glass transitions (Tg) at -63°C and -43°C.
The bulk water within the solution crystallized at -18°C (Tc).
Relaxation studies confirmed the presence of glassy states, showing characteristic overshoots at the observed glass transition temperatures.

Conclusions:

Aqueous d-ribose solutions can form more than one vitreous domain upon cooling to low temperatures.
The presence of multiple glass transitions suggests complex molecular arrangements and relaxation dynamics within the d-ribose-water system.
The crystallization of bulk water at -18°C is a significant thermal event distinct from the d-ribose-related glass transitions.

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