Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Simple method for differentiating between HLA and platelet-specific antibodies by flow cytometry.

J Freedman1, A Hornstein

  • 1Department of Immunohematology, St. Michael's Hospital, University of Toronto, Canada.

American Journal of Hematology
|December 1, 1991
PubMed
Summary

This study introduces a flow cytometry method using platelets and lymphocytes to detect antiplatelet antibodies. This technique helps differentiate between platelet-specific and HLA antibodies, improving accuracy in alloimmunized patients.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A phenome-wide association study of cross-disorder genetic liability in youth genetically similar to individuals from European reference populations.

Nature. Mental health·2026
Same author

Durvalumab and tremelimumab plus local partial tumour ablation (radiofrequency ablation or stereotactic radiotherapy) in patients with unresectable liver metastases from metastatic colorectal cancer: results of the EORTC-1560-GITCG multicentre, single-arm phase II study (ILOC).

ESMO open·2025
Same author

Phenome-wide Investigation of Behavioral, Environmental, and Neural Associations with Cross-Disorder Genetic Liability in Youth of European Ancestry.

medRxiv : the preprint server for health sciences·2023
Same author

Changes to country-specific hepatitis A travel vaccination recommendation for UK travellers in 2017-responding to a vaccine shortage in the national context.

Public health·2018
Same author

The impact of new national guidance for the public health management of enteric fever in England.

Public health·2017
Same author

High frequency jet ventilation for motion management during ablation procedures, a narrative review.

Acta anaesthesiologica Scandinavica·2017

Area of Science:

  • Immunology
  • Hematology
  • Transfusion Medicine

Background:

  • Platelets possess both platelet-specific and Human Leukocyte Antigen (HLA) Class I antigens.
  • Standard antiplatelet antibody detection methods may not distinguish between these specificities.
  • Accurate identification of antibody targets is crucial for transfusion safety.

Purpose of the Study:

  • To develop and validate a flow cytometry technique for detecting antiplatelet antibodies.
  • To differentiate between platelet-specific and HLA antibodies in patient sera.
  • To enhance the accuracy of platelet antibody identification in alloimmunized individuals.

Main Methods:

  • Utilizing flow cytometry with a mixed population of platelets and lymphocytes as target cells.
  • Employing citric acid or chloroquine treatment to modify surface HLA antigens on target cells.

Related Experiment Videos

  • Analyzing serum reactivity against both untreated and treated cell mixtures.

    • Main Results:

    • The mixed cell approach allows for confirmation of HLA antibodies in reactive sera.
    • Treatment methods aid in distinguishing platelet-specific antibodies from HLA antibodies.
    • The described technique shows potential for improved antiplatelet antibody detection.

    Conclusions:

    • A novel flow cytometry method using mixed platelets and lymphocytes enhances antiplatelet antibody detection.
    • This approach facilitates differentiation of antibody specificities, crucial for alloimmunized patients.
    • The technique may improve platelet crossmatching and transfusion strategies.