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Related Experiment Videos

Changes in glycoconjugate expression during early chick embryo development: a lectin-binding study.

C M Griffith1, E J Sanders

  • 1Department of Physiology, University of Alberta, Edmonton, Canada.

The Anatomical Record
|October 1, 1991
PubMed
Summary
This summary is machine-generated.

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Cell surface oligosaccharide changes occur during chick embryo gastrulation and neurulation. Lectin binding reveals alterations in epiblast and mesoderm cell surfaces, particularly involving the PNA-binding sequence during epithelial-mesenchymal transformation.

Area of Science:

  • Developmental Biology
  • Glycobiology
  • Cell Biology

Background:

  • Cell surface oligosaccharides play crucial roles in embryonic development.
  • Understanding changes in cell surface carbohydrates is key to deciphering developmental processes like gastrulation and neurulation.

Purpose of the Study:

  • To investigate developmentally regulated changes in cell surface oligosaccharide distribution during early chick embryo development.
  • To identify specific oligosaccharide modifications associated with gastrulation and neurulation stages.

Main Methods:

  • Utilized a selection of lectins with defined sugar specificities to probe cell surface carbohydrates.
  • Analyzed lectin binding patterns across different cell layers (epiblast, mesoderm, endoderm) during gastrulation and neurulation.

Related Experiment Videos

  • Employed ultrastructural analysis to determine the localization of lectin binding.

    • Main Results:

    • Distinct lectin binding patterns were observed in epiblast, mesoderm, and endoderm during gastrulation.
    • The primitive streak showed no lectin binding, while lateral epiblast regions indicated glucosamine residue loss.
    • Mesodermal cells exhibited significant lectin binding post-primitive streak passage, notably with peanut agglutinin (PNA), indicating changes in the Gal beta 1-3GalNAc sequence.
    • PNA binding was localized to extracellular matrix fibrils.

    Conclusions:

    • Epithelial-mesenchymal transformation during gastrulation involves significant cell surface oligosaccharide remodeling.
    • Specific carbohydrate changes, including the PNA-binding sequence, are associated with cell migration and differentiation.
    • Lectins are valuable tools for mapping dynamic changes in cell surface glycans during embryonic development.