Characterization of plasmid pASV479 from Bifidobacterium pseudolongum subsp. globosum and its use for expression

Related Experiment Video

Updated: Jul 15, 2026

A Seamless Cloning Approach for Porcine Reproductive and Respiratory Syndrome Virus Expression Vector Construction
04:18

A Seamless Cloning Approach for Porcine Reproductive and Respiratory Syndrome Virus Expression Vector Construction

Published on: May 17, 2024

A Sangrador-Vegas1, C Stanton, D van Sinderen

  • 1Moorepark Food Research Centre, Teagasc, Fermoy, County Cork, Ireland.

Plasmid
|May 1, 2007
PubMed

Insights

Researchers characterized the pASV479 plasmid from Bifidobacterium pseudolongum subsp. globosum DPC479. They developed a novel expression vector, pBIFRIBO, for use in bifidobacteria.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Bifidobacterium pseudolongum subsp. globosum DPC479 is an intestinal bacterium.
  • This strain harbors a 4.8 kb plasmid, designated pASV479.
  • Plasmids are crucial for genetic exchange and adaptation in bacteria.

Purpose of the Study:

  • To characterize the pASV479 plasmid.
  • To identify potential functional elements within the plasmid.
  • To construct a novel expression vector for bifidobacteria.

Main Methods:

  • Plasmid DNA isolation and characterization.
  • Bioinformatic analysis of open reading frames (ORFs).
  • Construction of an expression vector using the pASV479 backbone and a bifidobacteria rRNA promoter.

Main Results:

  • The pASV479 plasmid contains six open reading frames (ORFs).
  • Two ORFs show significant homology to replication and FtsK-like proteins from Bifidobacterium breve plasmids, suggesting relatedness.
  • A functional expression vector, pBIFRIBO, was successfully constructed.

Conclusions:

  • The pASV479 plasmid possesses genetic elements related to other bifidobacterial plasmids.
  • The constructed pBIFRIBO vector provides a new tool for genetic manipulation in bifidobacteria.
  • This work facilitates further research into bifidobacterial gene function and applications.

More Related Videos

Generation of Plasmid Vectors Expressing FLAG-tagged Proteins Under the Regulation of Human Elongation Factor-1α Promoter Using Gibson Assembly10:18

Generation of Plasmid Vectors Expressing FLAG-tagged Proteins Under the Regulation of Human Elongation Factor-1α Promoter Using Gibson Assembly

Published on: February 9, 2015

High-Resolution Comparison of Bacterial Conjugation Frequencies05:18

High-Resolution Comparison of Bacterial Conjugation Frequencies

Published on: January 10, 2019

Related Concept Videos

Plasmids01:28

Plasmids

Plasmids are extrachromosomal DNA molecules found in bacteria, archaea, and some eukaryotic microbes like yeast. These small, circular DNA structures typically contain fewer than 30 genes, although some may exist linearly. Plasmids vary in their number within a cell, known as copy number. Single-copy plasmids are present in one copy per cell and multi-copy plasmids are present in multiple copies, reaching over 100 copies per cell.Plasmids usually replicate independently of the chromosomal DNA...
DNA Bacteriophages01:26

DNA Bacteriophages

Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...
Conjugation01:19

Conjugation

Conjugation is a form of horizontal gene transfer that primarily occurs in bacteria and some archaea, promoting genetic diversity and adaptation. Bacteria can acquire resistance genes through conjugative plasmids, allowing them to survive antibiotic treatments that would otherwise be lethal. This process involves direct contact between cells through specialized structures such as the sex pilus and is mediated by conjugative plasmids, including the F (fertility) factor.Conjugation requires...