Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Non-LTR Retrotransposons03:18

Non-LTR Retrotransposons

As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
LTR Retrotransposons03:08

LTR Retrotransposons

LTR retrotransposons are class I transposable elements with long terminal repeats flanking an internal coding region. These elements are less abundant in mammals compared to other class I transposable elements. About 8 percent of human genomic DNA comprises LTR retrotransposons. Some of the common examples of LTR retrotransposons are Ty elements in yeast and Copia elements in Drosophila.
The internal coding region of LTR retrotransposons and their mechanism of transposition closely resembles a...
Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for injury repair.
Embryonic Stem Cells00:57

Embryonic Stem Cells

Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
Embryonic Stem Cells00:58

Embryonic Stem Cells

Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Transcriptional perturbation of LINE-1 elements reveals their cis-regulatory potential.

EMBO reports·2026
Same author

Single-cell proteomics: When individual cells tell population stories.

Trends in biochemical sciences·2026
Same author

Brain Organoids, Lessons from Fetal Neocortex Formation, and Rational Design for Quality Control.

bioRxiv : the preprint server for biology·2026
Same author

A gene therapy strategy for Deoxyhypusine Synthase (DHPS) syndrome.

Research square·2026
Same author

SETD5 dysfunction in human astrocytes drives IL-6-mediated neuronal impairments via the JAK/STAT signaling pathway.

bioRxiv : the preprint server for biology·2026
Same author

Reconstructing human corticogenesis: Insights from cerebral organoids into neurodevelopment and disease modeling.

Developmental cell·2026

Related Experiment Video

Updated: Jul 15, 2026

Analysis of LINE-1 Retrotransposition at the Single Nucleus Level
11:52

Analysis of LINE-1 Retrotransposition at the Single Nucleus Level

Published on: April 23, 2016

LINE-1 retrotransposition in human embryonic stem cells.

Jose L Garcia-Perez1, Maria C N Marchetto, Alysson R Muotri

  • 1Departments of Human Genetics and Internal Medicine, University of Michigan Medical School, 1241 E. Catherine Street, Ann Arbor, MI 48109, USA.

Human Molecular Genetics
|May 1, 2007
PubMed
Summary

Human embryonic stem cells can support LINE-1 retrotransposition, a mobile DNA element impacting genome evolution. These events may occur during early human development, potentially affecting gene function and DNA integrity.

More Related Videos

Retroviral Infection of Murine Embryonic Stem Cell Derived Embryoid Body Cells for Analysis of Hematopoietic Differentiation
11:40

Retroviral Infection of Murine Embryonic Stem Cell Derived Embryoid Body Cells for Analysis of Hematopoietic Differentiation

Published on: October 20, 2014

RNA Next-Generation Sequencing and a Bioinformatics Pipeline to Identify Expressed LINE-1s at the Locus-Specific Level
11:04

RNA Next-Generation Sequencing and a Bioinformatics Pipeline to Identify Expressed LINE-1s at the Locus-Specific Level

Published on: May 19, 2019

Related Experiment Videos

Last Updated: Jul 15, 2026

Analysis of LINE-1 Retrotransposition at the Single Nucleus Level
11:52

Analysis of LINE-1 Retrotransposition at the Single Nucleus Level

Published on: April 23, 2016

Retroviral Infection of Murine Embryonic Stem Cell Derived Embryoid Body Cells for Analysis of Hematopoietic Differentiation
11:40

Retroviral Infection of Murine Embryonic Stem Cell Derived Embryoid Body Cells for Analysis of Hematopoietic Differentiation

Published on: October 20, 2014

RNA Next-Generation Sequencing and a Bioinformatics Pipeline to Identify Expressed LINE-1s at the Locus-Specific Level
11:04

RNA Next-Generation Sequencing and a Bioinformatics Pipeline to Identify Expressed LINE-1s at the Locus-Specific Level

Published on: May 19, 2019

Area of Science:

  • Genetics
  • Genomics
  • Developmental Biology

Background:

  • LINE-1 elements constitute a significant portion of the human genome and are known for their mobility.
  • The role of non-transformed cells, particularly stem cells, in supporting LINE-1 retrotransposition remains largely unexplored.

Purpose of the Study:

  • To investigate the capacity of human embryonic stem cells (hESCs) to support LINE-1 retrotransposition.
  • To characterize the nature and consequences of LINE-1 retrotransposition events in hESCs.

Main Methods:

  • Culturing and maintaining human embryonic stem cells in vitro.
  • Detecting and analyzing endogenous LINE-1 element expression and activity.
  • Sequencing and analyzing retrotransposition insertion sites and associated genomic alterations.

Main Results:

  • Human embryonic stem cells express endogenous LINE-1 elements.
  • hESCs can accommodate LINE-1 retrotransposition in vitro.
  • Retrotransposition events were observed to insert into genes and cause deletions at target sites.

Conclusions:

  • Human embryonic stem cells are capable of supporting LINE-1 retrotransposition.
  • LINE-1 retrotransposition may occur during early human development.
  • These events have the potential to impact gene function and genomic stability during development.