Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Damage can Stall the Cell Cycle02:36

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
DNA Damage Can Stall the Cell Cycle02:36

DNA Damage Can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
Centrosome Duplication02:25

Centrosome Duplication

The primary microtubule organizing center (MTOC) in animal cells is the centrosome. A centrosome has two cylindrical centrioles at its core. Each centriole consists of nine sets of three microtubules held together by proteins. The centrioles are positioned at right angles to each other and surrounded by a shapeless protein cloud called the pericentriolar matrix, or pericentriolar material (PCM).
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
Centrosome Duplication02:25

Centrosome Duplication

The primary microtubule organizing center (MTOC) in animal cells is the centrosome. A centrosome has two cylindrical centrioles at its core. Each centriole consists of nine sets of three microtubules held together by proteins. The centrioles are positioned at right angles to each other and surrounded by a shapeless protein cloud called the pericentriolar matrix, or pericentriolar material (PCM).
To ensure that each daughter cell receives a centrosome after cell division, centrosome duplication...
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
Nucleotide Excision Repair01:38

Nucleotide Excision Repair

DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evaluating PrEP equity in Wales, UK, using triangulation of routine data sources.

Sexually transmitted infections·2026
Same author

The co-existence of Ehlers-Danlos syndrome and postural orthostatic tachycardia syndrome: A systematic review of the literature.

Autonomic neuroscience : basic & clinical·2026
Same author

Risk of dementia after bloodstream infection-a nationwide propensity score matched cohort study.

Age and ageing·2026
Same author

Responsible preoperative opioid use for hip or knee arthroplasty (OpioidHALT): a protocol for a randomised clinical trial of pharmacist-partnered opioid tapering prior to hip or knee arthroplasty.

BMJ open·2026
Same author

Decision on Optimal Combinatorial Therapies in Immune-mediated inflammatory diseases using Systems approaches (DocTIS): protocol for a single-arm, adaptive basket trial in rheumatoid and psoriatic arthritis.

Rheumatology advances in practice·2026
Same author

Comprehensive analysis of TEAD inhibition in meningioma identifies MEK and mTOR inhibition as effective combination therapies against resistant lines.

bioRxiv : the preprint server for biology·2026

Related Experiment Video

Updated: Jul 15, 2026

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage
10:59

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage

Published on: August 21, 2021

DNA damage induces Chk1-dependent centrosome amplification.

Emer Bourke1, Helen Dodson, Andreas Merdes

  • 1Department of Biochemistry and NCBES, National University of Ireland-Galway, University Road, Galway, Ireland.

EMBO Reports
|May 1, 2007
PubMed
Summary

DNA damage causes centrosome amplification, not fragmentation, in human cells. Checkpoint kinase 1 (Chk1) activity is crucial for this process following DNA damage.

More Related Videos

Study of the DNA Damage Checkpoint using Xenopus Egg Extracts
10:55

Study of the DNA Damage Checkpoint using Xenopus Egg Extracts

Published on: November 5, 2012

Two- and Three-Dimensional Live Cell Imaging of DNA Damage Response Proteins
10:24

Two- and Three-Dimensional Live Cell Imaging of DNA Damage Response Proteins

Published on: September 28, 2012

Related Experiment Videos

Last Updated: Jul 15, 2026

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage
10:59

Visualizing and Quantifying Endonuclease-Based Site-Specific DNA Damage

Published on: August 21, 2021

Study of the DNA Damage Checkpoint using Xenopus Egg Extracts
10:55

Study of the DNA Damage Checkpoint using Xenopus Egg Extracts

Published on: November 5, 2012

Two- and Three-Dimensional Live Cell Imaging of DNA Damage Response Proteins
10:24

Two- and Three-Dimensional Live Cell Imaging of DNA Damage Response Proteins

Published on: September 28, 2012

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Cancer Research

Background:

  • Centrosomal abnormalities are common in cancer and DNA repair deficiencies.
  • Understanding the mechanisms linking DNA damage to centrosome abnormalities is critical.

Purpose of the Study:

  • To investigate the relationship between DNA damage and centrosome amplification in human cells.
  • To elucidate the roles of ATM, ATR, and Chk1 in DNA-damage-induced centrosome amplification.

Main Methods:

  • Light and electron microscopy were employed to observe centrosome morphology.
  • Genetic and pharmacological inhibition of ATM, ATR, and Chk1 pathways were utilized.
  • RNA-mediated interference and drug treatments were used to inhibit Chk1.
  • Analysis of centrosome amplification in Chk1-deficient cells with and without Chk1 expression.

Main Results:

  • DNA damage induces centrosome amplification, not fragmentation, in human cells.
  • Caffeine treatment abrogated amplification in ATM- and ATR-defective cells, suggesting complementary roles for these kinases.
  • Inhibition of checkpoint kinase 1 (Chk1) suppressed DNA-damage-induced centrosome amplification.
  • Radiation-induced centrosome amplification was abolished in Chk1-deficient cells, but restored by Chk1 expression.
  • Catalytic activity and signaling to Chk1 are essential for promoting centrosome overduplication after DNA damage.

Conclusions:

  • DNA damage triggers centrosome amplification through a pathway involving ATM, ATR, and Chk1.
  • Chk1 catalytic activity and proper signaling are indispensable for DNA-damage-induced centrosome overduplication.
  • These findings highlight Chk1 as a key regulator in the response of centrosomes to DNA damage, with implications for cancer biology.