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Updated: Apr 25, 2026
RNA Next-Generation Sequencing and a Bioinformatics Pipeline to Identify Expressed LINE-1s at the Locus-Specific Level
Published on: May 19, 2019
Expressed sequenced tags from Lygus lineolaris (Hemiptera: Miridae), the tarnished plant bug
1Biological Control of Pests Research Unit, US Department of Agriculture, Agricultural Research Service, Stoneville, MS, USA. MLAllen@msa-stoneville.ars.usda.gov
Abstract:
Expressed sequenced tags (ESTs) were prepared to establish a baseline for molecular genetic studies of the tarnished plant bug, Lygus lineolaris (Palisot de Beauvois). The largest class of identifiable ESTs (15.2%) was from genes involved in cellular metabolic functions, including physiological processes. Twenty-seven ESTs (9.8%) were from genes associated with transcription and translation, including ribosomal genes. One hundred and forty-two of the 276 unique ESTs were from genes not previously identified from any organism. Twelve sequences appear to be associated with feeding and digestion and may be targets for pest control studies.
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