Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Genome integrity: a HAT needs a chaperone.

Craig L Peterson1

  • 1Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA. craig.peterson@umassmed.edu

Current Biology : CB
|May 2, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

CFDP1 is required for histone variant H2A.Z deposition by the human SRCAP chromatin remodeling complex.

bioRxiv : the preprint server for biology·2025
Same author

Dynamic Conformations of Chromatin Remodeler ISWI during Nucleosome Sliding Revealed by Hydrogen-Deuterium Exchange Coupled to Mass Spectrometry.

Biochemistry·2025
Same author

Heterochromatin: Hiding from the remodeling machines.

Molecular cell·2024
Same author

Dual engagement of the nucleosomal acidic patches is essential for deposition of histone H2A.Z by SWR1C.

eLife·2024
Same author

Dual engagement of the nucleosomal acidic patches is essential for deposition of histone H2A.Z by SWR1C.

Research square·2023
Same author

Cryo-EM structure of the human Sirtuin 6-nucleosome complex.

Science advances·2023
Same journal

Hunting ecology predicts eye arrangements in the modular visual system of spiders.

Current biology : CB·2026
Same journal

Sub-second fluctuations between top-down and bottom-up modes distinguish diverse human brain states.

Current biology : CB·2026
Same journal

Queen bees offload pesticide burden to eggs when social buffering is overwhelmed.

Current biology : CB·2026
Same journal

Pitch selectivity in ferret auditory cortex.

Current biology : CB·2026
Same journal

A cell size-dependent competition between geometry and polarity governs nuclear and spindle positioning in early embryos.

Current biology : CB·2026
Same journal

Trophic cascades drive sustainability in the agricultural heritage rice-fish coculture system.

Current biology : CB·2026
See all related articles

Histone acetylation maintains yeast replisome integrity against genotoxic agents. Protein Rtt109p is identified as the catalytic subunit of a histone H3 acetylase complex crucial for this process.

Area of Science:

  • Molecular Biology
  • Yeast Genetics
  • Epigenetics

Background:

  • Histone acetylation plays a role in maintaining genome stability.
  • The yeast replisome is essential for DNA replication and integrity.
  • Genotoxic agents pose a threat to cellular DNA.

Purpose of the Study:

  • To identify the key protein responsible for histone acetylation in yeast.
  • To characterize the function of protein Rtt109p in maintaining replisome integrity.
  • To elucidate the composition and activity of the histone H3 acetylase complex.

Main Methods:

  • Genetic analysis in yeast models.
  • Biochemical assays to determine enzyme activity.
  • Protein complex purification and characterization.

Related Experiment Videos

Main Results:

  • Protein Rtt109p was identified as the catalytic subunit of a novel histone H3 acetylase.
  • A histone chaperone subunit was found to be essential for the complex's activity.
  • This complex is critical for maintaining yeast replisome integrity.

Conclusions:

  • Rtt109p is a key enzyme in the histone acetylation pathway.
  • The identified histone H3 acetylase complex is vital for DNA repair and replication.
  • Understanding this complex provides insights into genome stability mechanisms.