Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

Peptide Identification Using Tandem Mass Spectrometry

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...

Rapid Identification of Pathogens

Rapid Identification of Pathogens

MALDI-TOF MS has transformed clinical microbiology by offering a rapid and reliable method for pathogen identification. The traditional approach to microbial identification typically involves time-consuming culture techniques and biochemical tests, which can delay the initiation of appropriate antimicrobial therapy. MALDI-TOF MS avoids these delays by using characteristic ribosomal protein mass patterns of microbial cells, enabling accurate species-level identification within minutes.Principle...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Abnormal Brain Iron Metabolism is Linked to Altered Neural Function in Isolated Laryngeal Dystonia.

Movement disorders : official journal of the Movement Disorder Society·2025

Same author

Characterization of Microscopic Multicellular Foci in Grossly Normal Renal Parenchyma of Von Hippel-Lindau Kidney.

Medicina (Kaunas, Lithuania)·2022

Same author

Kidney in VHL disease: Early clear cell proliferation occurs in the distal tubular system.

Oncology reports·2022

Same author

Developmentally Arrested Basket/Stellate Cells in Postnatal Human Brain as Potential Tumor Cells of Origin for Cerebellar Hemangioblastoma in von Hippel-Lindau Patients.

Journal of neuropathology and experimental neurology·2022

Same author

Qualitative Comparison of Cryostat- versus Snap-Frozen Neurosurgical Intraoperative Consultations.

International journal of surgical pathology·2022

Same author

Multimodal Atlas of the Murine Inner Ear: From Embryo to Adult.

Frontiers in neurology·2021

Search research articles

Related Experiment Video

Updated: Jul 15, 2026

A High Throughput, Multiplexed and Targeted Proteomic CSF Assay to Quantify Neurodegenerative Biomarkers and Apolipoprotein E Isoforms Status

A High Throughput, Multiplexed and Targeted Proteomic CSF Assay to Quantify Neurodegenerative Biomarkers and Apolipoprotein E Isoforms Status

Published on: October 20, 2016

Proteomic profiling: a novel method for differential diagnosis?

Sven Gläsker¹, Russell R Lonser, Hiroaki Okamoto

¹Surgical Neurology Branch, National Institutes of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, Maryland 20892, USA. sven.glaeskar@uniklinik.freiburg.de

Cancer Biology & Therapy

|May 2, 2007

Summary

Distinguishing brain hemangioblastomas from metastatic renal cell carcinoma (RCC) can be challenging. Proteomic profiling successfully identified a cerebellar tumor as RCC metastasis, not hemangioblastoma, aiding diagnosis.

More Related Videos

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors

Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors

Published on: February 27, 2015

Related Experiment Videos

Last Updated: Jul 15, 2026

A High Throughput, Multiplexed and Targeted Proteomic CSF Assay to Quantify Neurodegenerative Biomarkers and Apolipoprotein E Isoforms Status

A High Throughput, Multiplexed and Targeted Proteomic CSF Assay to Quantify Neurodegenerative Biomarkers and Apolipoprotein E Isoforms Status

Published on: October 20, 2016

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors

Quantitative Mass Spectrometric Profiling of Cancer-cell Proteomes Derived From Liquid and Solid Tumors

Published on: February 27, 2015

Area of Science:

Neuro-oncology
Pathology
Proteomics

Background:

Differentiating benign hemangioblastoma from metastatic renal cell carcinoma (RCC) in the brain presents a diagnostic challenge due to overlapping phenotypes.
Patients with von Hippel-Lindau disease and known RCC are particularly susceptible to this diagnostic quandary.

Observation:

A 59-year-old female with von Hippel-Lindau disease and RCC presented with a cystic cerebellar tumor with imaging suggestive of hemangioblastoma.
Histological examination revealed features ambiguous between hemangioblastoma and atypical RCC metastasis.
Conventional clinical and pathological methods failed to definitively resolve the diagnostic discrepancy.

Findings:

Proteomic profiling, utilizing selective tissue microdissection and 2D gel electrophoresis, was employed to compare the unknown lesion with known hemangioblastoma and RCC samples.
The proteomic expression pattern of the cerebellar tumor strongly indicated a diagnosis of renal cell carcinoma metastasis.
This molecular analysis successfully clarified the diagnostic dilemma posed by the ambiguous histology.

Implications:

Proteomics offers a valuable tool to complement traditional histopathology in complex tumor characterization and diagnosis.
Metastatic renal cell carcinoma does not always exhibit aggressive growth patterns, challenging conventional assumptions about malignant tumor behavior.
This case highlights the importance of advanced molecular techniques in refining differential diagnoses for brain tumors.