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Related Experiment Video

Updated: Jul 15, 2026

Fluorescence detection methods for microfluidic droplet platforms
14:16

Fluorescence detection methods for microfluidic droplet platforms

Published on: December 10, 2011

Quantitative kinetic analysis in a microfluidic device using frequency-domain fluorescence lifetime imaging.

Sinéad M Matthews1, Alan D Elder, Kamran Yunus

  • 1Department of Chemical Engineering, University of Cambridge, New Museums Site, Pembroke Street, Cambridge, CB2 3RA, UK.

Analytical Chemistry
|May 3, 2007
PubMed
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This study introduces a new microfluidic method using numerical simulation and fluorescence lifetime imaging to quantify chemical reaction rates. The technique successfully determined reaction kinetics, including diffusion and rate constants.

Area of Science:

  • Chemical kinetics
  • Microfluidics
  • Fluorescence imaging

Background:

  • Quantifying reaction kinetics in microfluidic devices is challenging.
  • Fluorescence lifetime imaging (FLI) offers potential for in-situ measurements.
  • Integrating FLI with numerical simulations can enhance kinetic analysis.

Purpose of the Study:

  • To develop and validate a novel microfluidic approach for quantifying reaction kinetics.
  • To extract quantitative kinetic information from fluorescence lifetime imaging data using numerical simulations.
  • To demonstrate the method's applicability to both diffusion and complex chemical reactions.

Main Methods:

  • A three-dimensional finite difference numerical simulation was developed.
  • Fluorescence lifetime imaging experimental data was analyzed using the simulation.

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Related Experiment Videos

Last Updated: Jul 15, 2026

Fluorescence detection methods for microfluidic droplet platforms
14:16

Fluorescence detection methods for microfluidic droplet platforms

Published on: December 10, 2011

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells
09:45

Fluorescence Lifetime Imaging of Molecular Rotors in Living Cells

Published on: February 9, 2012

Advanced Compositional Analysis of Nanoparticle-polymer Composites Using Direct Fluorescence Imaging
07:41

Advanced Compositional Analysis of Nanoparticle-polymer Composites Using Direct Fluorescence Imaging

Published on: July 19, 2016

  • The method was applied to a fluorescence quenching reaction to map quencher diffusion.
  • The approach was used to determine the bimolecular rate constant of an amine-acid chloride reaction.

    • Main Results:

    • The microfluidic approach successfully quantified reaction kinetics.
    • Diffusion of a quencher across a microchannel was mapped using fluorophore lifetime.
    • The bimolecular rate constant for a complex chemical reaction was accurately obtained via numerical simulation.

    Conclusions:

    • The presented microfluidic approach combined with numerical simulation and FLI is effective for quantitative kinetic studies.
    • This method provides a powerful tool for analyzing reaction dynamics in microscale environments.
    • The technique is versatile, applicable to diffusion processes and complex chemical reactions.