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Updated: Jul 15, 2026

Autofluorescence Imaging to Evaluate Red Algae Physiology
Published on: February 17, 2023
Microimaging of algal bioconvection by scanning electrochemical microscopy
Isao Shitanda1, Yutaka Yoshida, Tetsu Tatsuma
1Institute of Industrial Science, University of Tokyo, Komaba, Meguro-ku, Tokyo 153-8505, Japan.
Abstract:
Local bioconvection generated by algal flagellar movement was imaged by scanning electrochemical microscopy. As a microelectrode probe vertically approached an individual multicellular flagellate alga, Volvox carteri, an oxidation current of a coexisting redox marker ([Fe(CN)6]4-) increased gradually, due to bioconvective enhancement of mass transport, and eventually decreased because the algal body blocked the diffusion of the marker. Two-dimensional imaging of the bioconvection of an individual alga was also possible. The bioconvective enhancement of the current was hindered by a toxic compound that inhibits the flagellar movement.
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