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Updated: Jul 15, 2026
06:34
Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
Published on: July 11, 2016
Flavobacterium terrigena sp. nov., isolated from soil
Jung-Hoon Yoon1, So-Jung Kang1, Jung-Sook Lee1
1Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Korea.
Summary
A novel bacterial species, Flavobacterium terrigena, was discovered in Korean soil. This Flavobacterium-like strain, DS-20(T), represents a new species based on genetic and phenotypic analysis.
Area of Science:
- Microbiology
- Bacteriology
- Taxonomy
Background:
- Soil microbial communities harbor diverse bacterial species.
- The genus Flavobacterium includes Gram-negative, rod-shaped bacteria with various ecological roles.
- Accurate bacterial classification is crucial for understanding microbial diversity and function.
Purpose of the Study:
- To characterize a novel bacterial isolate from Dokdo soil.
- To determine the phylogenetic and phenotypic distinctiveness of strain DS-20(T).
- To formally propose a new species within the genus Flavobacterium.
Main Methods:
- Polyphasic taxonomic study including phenotypic analysis.
- Determination of optimal growth conditions (pH, temperature).
- Analysis of cellular fatty acids and menaquinone composition.
- Phylogenetic analysis based on 16S rRNA gene sequencing.
- DNA G+C content determination.
Main Results:
- Strain DS-20(T) is a Gram-negative, rod-shaped bacterium.
- Optimal growth observed at pH 6.5-7.0 and 25°C.
- Predominant menaquinone: MK-6; major fatty acids: iso-C15:0, iso-C17:0 3-OH, iso-C17:1ω9c.
- DNA G+C content: 38.2 mol%.
- 16S rRNA gene sequence similarity to related Flavobacterium species < 94.9%.
- Phenotypic characteristics differentiate strain DS-20(T) from known species.
Conclusions:
- Strain DS-20(T) represents a novel species within the genus Flavobacterium.
- The proposed name for this new species is Flavobacterium terrigena sp. nov.
- The type strain is DS-20(T) (=KCTC 12761(T)=DSM 17934(T)).
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