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Updated: Jul 15, 2026

Near Infrared Optical Projection Tomography for Assessments of β-cell Mass Distribution in Diabetes Research

Near Infrared Optical Projection Tomography for Assessments of β-cell Mass Distribution in Diabetes Research

Published on: January 12, 2013

Resolution improvement in emission optical projection tomography.

Johnathon R Walls¹, John G Sled, James Sharpe

¹Mouse Imaging Centre, Hospital for Sick Children, University of Toronto, 555 University Avenue, Toronto, ON M5G 1X8, Canada. johnathon.walls@utoronto.ca

Physics in Medicine and Biology

|May 3, 2007

Summary

A new imaging method, emission optical projection tomography (eOPT), enhances molecular imaging in embryos. A novel frequency space filter significantly reduces image blurring, improving resolution for detailed studies.

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Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

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Published on: August 16, 2012

Area of Science:

Biomedical Imaging
Molecular Imaging
Developmental Biology

Background:

Emission optical projection tomography (eOPT) offers molecular specificity and high resolution for embryo imaging.
Image blurring in eOPT, particularly at greater distances from the rotation axis, limits its application.
This blurring is partly due to lens defocusing, which is dependent on object distance.

Purpose of the Study:

To develop and validate a frequency space filter to reduce blurring in eOPT images.
To improve the resolution and clarity of eOPT reconstructions for gene expression studies in embryos.

Main Methods:

A frequency space filter was designed based on the frequency-distance relationship in sinograms.
The filter deconvolves the distance-dependent point-spread function.
Highly defocused data was excluded from eOPT sinograms before image reconstruction.

Main Results:

The filtering method significantly reduced blurring in reconstructed eOPT images.
The volume at half-maximum of the point-spread function was reduced to approximately 20% of the original.
The volume at 10% maximum was reduced to approximately 6% of the original, dramatically improving detail visibility.

Conclusions:

The developed frequency space filter effectively mitigates blurring in eOPT.
This technique enhances the resolution and detail in molecular imaging of biological specimens, particularly embryos.
Improved eOPT imaging facilitates more accurate gene expression studies and developmental biology research.