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Updated: Jul 15, 2026
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Intrastriatal Injection of Autologous Blood or Clostridial Collagenase as Murine Models of Intracerebral Hemorrhage
Published on: July 3, 2014
Experimental intracerebral hematoma in the rat
Alain Barth1, Raphael Guzman, Robert H Andres
1Department of Neurosurgery, University Hospital of Bern, CH-3010 Bern, Switzerland. alain.barth@meduni-graz.at
Restorative Neurology and Neuroscience
|May 3, 2007
Summary
This study presents a refined rat model for intracerebral hemorrhage, improving reproducibility and reducing mortality for neurotransplantation research. The new method ensures consistent hematoma size and location, preserving surrounding brain tissue.
Area of Science:
- Neuroscience
- Surgical Models
- Animal Research
Background:
- Current intracerebral hemorrhage models exhibit high mortality and variability.
- These limitations hinder their use in neurotransplantation studies.
Purpose of the Study:
- To develop a modified autologous blood infusion technique in rats.
- To reduce limitations of existing models for intracerebral hemorrhage research.
Main Methods:
- A mechanical microlesion was created stereotactically in the rat striatum before blood infusion.
- A rotating microcatheter facilitated parenchyma lesioning and slow infusion of autologous blood.
- Hematoma volume, morphology, and behavioral deficits were quantified.
Main Results:
- The modified model demonstrated zero surgical mortality and highly reproducible hematoma volumes (15.20 ± 0.60 mm³).
- Hematoma edges were sharply delineated with preserved perihematomal tissue.
- Animals exhibited initial deficits in spontaneous rotation and persistent forelimb placing deficits.
Conclusions:
- This model offers systematic control and high reproducibility in hematoma production.
- It preserves critical perihematomal brain tissue.
- The model yields quantifiable neurological deficits, suitable for research.
