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Related Concept Videos

Conserved Binding Sites01:49

Conserved Binding Sites

Many proteins’ biological role depends on their interactions with their ligands, small molecules that bind to specific locations on the protein known as ligand-binding sites. Ligand-binding sites are often conserved among homologous proteins as these sites are critical for protein function.
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally analyses the...
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form dimers that...
Conserved Binding Sites01:49

Conserved Binding Sites

Many proteins’ biological role depends on their interactions with their ligands, small molecules that bind to specific locations on the protein known as ligand-binding sites. Ligand-binding sites are often conserved among homologous proteins as these sites are critical for protein function.
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally analyses the...
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form dimers that...

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Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
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Published on: April 26, 2017

RNA-binding proteins: modular design for efficient function.

Bradley M Lunde1, Claire Moore, Gabriele Varani

  • 1Department of Biochemistry, University of Washington, Seattle, Washington 98195, USA.

Nature Reviews. Molecular Cell Biology
|May 3, 2007
PubMed
Summary
This summary is machine-generated.

RNA-binding proteins utilize modular domains for diverse functions. These cooperating modules dictate RNA specificity and biological activity, forming the core functional unit.

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Last Updated: Jun 27, 2026

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Published on: April 26, 2017

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins
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Published on: August 9, 2019

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Area of Science:

  • Molecular Biology
  • Biochemistry
  • Structural Biology

Background:

  • Many RNA-binding proteins (RBPs) possess modular structures.
  • These proteins are built from repeating functional domains arranged in various configurations.
  • This modularity allows RBPs to fulfill diverse biological roles.

Purpose of the Study:

  • To investigate the cooperative mechanisms of different modules within RBPs.
  • To understand how these modules regulate RNA-binding specificity and protein activity.
  • To explore the interplay between modular domains and enzymatic domains in RNA-modifying enzymes.

Main Methods:

  • Analysis of protein structures and domain arrangements.
  • Investigating functional cooperation between protein modules.
  • Studying the impact of modularity on RNA binding and enzymatic activity.

Main Results:

  • Multiple domains cooperate to determine RNA-binding specificity.
  • Cooperation between modules influences the overall biological activity of RBPs.
  • Modular arrangements are crucial for regulating catalytic activity in RNA-acting enzymes.

Conclusions:

  • The fundamental structural unit responsible for RBP biological function is defined by multiple cooperating modules.
  • Understanding RBP modularity is key to deciphering their diverse roles in RNA regulation.