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Updated: Jul 15, 2026

Understanding Early Organogenesis Using a Simplified In Situ Hybridization Protocol in Xenopus

Understanding Early Organogenesis Using a Simplified In Situ Hybridization Protocol in Xenopus

Published on: January 12, 2015

Xenopus cDNA microarray identification of genes with endodermal organ expression.

Edmond Changkyun Park¹, Tadayoshi Hayata, Ken W Y Cho

¹Division of Molecular and Life Sciences, Pohang University of Science and Technology, Kyungbuk, Republic of Korea.

Developmental Dynamics : an Official Publication of the American Association of Anatomists

|May 3, 2007

Summary

Researchers identified 104 Xenopus endodermal genes crucial for organ development. These genes serve as vital markers for understanding how the endoderm forms digestive and respiratory organs during organogenesis.

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Last Updated: Jul 15, 2026

Understanding Early Organogenesis Using a Simplified In Situ Hybridization Protocol in Xenopus

Understanding Early Organogenesis Using a Simplified In Situ Hybridization Protocol in Xenopus

Published on: January 12, 2015

Functional Cloning Using a Xenopus Oocyte Expression System

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Published on: January 30, 2016

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Published on: February 2, 2016

Area of Science:

Developmental Biology
Genetics

Background:

The endoderm, a primary germ layer, forms vital organs like the liver, pancreas, and lungs.
The precise molecular mechanisms governing endodermal organogenesis remain incompletely understood.

Purpose of the Study:

To investigate the molecular basis of endodermal organogenesis.
To identify novel endodermal organ-related marker genes in Xenopus.

Main Methods:

Microarray analysis was performed on Xenopus gut endoderm from three developmental stages.
Xenopus gut endoderm was dissected into anterior and posterior regions for comparative analysis.
Competitive hybridization identified genes with specific expression patterns in major organs.

Main Results:

915 candidate genes were initially identified through differential screening.
104 candidate genes were selected for further analysis via in situ hybridization.
The expression patterns of these 104 genes during Xenopus organogenesis were characterized.

Conclusions:

This study successfully identified 104 Xenopus endodermal genes.
These genes represent valuable markers for studying endodermal organ development and organogenesis.
The findings provide a foundation for future research into the molecular mechanisms of organ formation.