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Updated: Jul 15, 2026

Chronic, Acute, and Reactivated HIV Infection in Humanized Immunodeficient Mouse Models
09:54

Chronic, Acute, and Reactivated HIV Infection in Humanized Immunodeficient Mouse Models

Published on: December 3, 2019

Primary cell model for activation-inducible human immunodeficiency virus.

Bryan Burke1, Helen J Brown, Matthew D Marsden

  • 1Department of Microbiology, Immunology, and Molecular Genetics, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA 90095-1678, USA.

Journal of Virology
|May 4, 2007
PubMed
Summary

Scientists developed a new in vitro model for dormant human immunodeficiency virus (HIV) infection in primary human cells. This model helps study HIV latency and activation, crucial for eliminating the viral reservoir.

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Area of Science:

  • Virology
  • Immunology
  • Cell Biology

Background:

  • Quiescent T lymphocytes harbor latent human immunodeficiency virus (HIV), forming a persistent viral reservoir.
  • This latent reservoir can reinitiate active infection after antiretroviral therapy cessation.
  • Understanding HIV latency mechanisms is vital for developing strategies to eradicate the viral reservoir.

Purpose of the Study:

  • To establish a novel in vitro model for dormant HIV infection in primary human cells.
  • To recapitulate key aspects of latent HIV infection for further study.
  • To provide a tool for investigating activation-inducible HIV infection and long terminal repeat regulation.

Main Methods:

  • Generation of a stable HIV infection in primary human CD4(+) CD8(+) thymocytes using a reporter virus.

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Humanized NOD/SCID/IL2rγnull (hu-NSG) Mouse Model for HIV Replication and Latency Studies
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Last Updated: Jul 15, 2026

Chronic, Acute, and Reactivated HIV Infection in Humanized Immunodeficient Mouse Models
09:54

Chronic, Acute, and Reactivated HIV Infection in Humanized Immunodeficient Mouse Models

Published on: December 3, 2019

Stem-cell Based Engineered Immunity Against HIV Infection in the Humanized Mouse Model
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Stem-cell Based Engineered Immunity Against HIV Infection in the Humanized Mouse Model

Published on: July 2, 2016

Humanized NOD/SCID/IL2rγnull (hu-NSG) Mouse Model for HIV Replication and Latency Studies
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Humanized NOD/SCID/IL2rγnull (hu-NSG) Mouse Model for HIV Replication and Latency Studies

Published on: January 7, 2019

  • Assessment of viral gene expression in the absence of T-cell activation.
  • Induction of reporter activity upon T-cell activation and analysis of the integrated viral genome.

    • Main Results:

    • A stable, dormant HIV infection was established in primary human thymocytes without viral gene expression.
    • T-cell activation induced a significant (>200-fold) increase in reporter activity.
    • The induced reporter activity stemmed from a fully reverse-transcribed and integrated viral genome.
    • NF-kappaB response element mutations were shown to decrease the activation of viral gene expression.

    Conclusions:

    • The developed in vitro model effectively recapitulates key features of dormant HIV infection in primary human cells.
    • This model allows for the study of activation-inducible HIV gene expression and long terminal repeat regulation.
    • The model serves as a valuable tool for investigating strategies to eliminate the latent HIV reservoir.